萱草花粉中微管蛋白生物化学性质  

Biochemical Characterization of the Pollen Tubulin from Day Lily (Hemerocallis fulva,Liliaceae)

作　　者：廖俊杰[1] 吴英杰 阎隆飞[1] 

机构地区：[1]中国农业大学生物学院植物生理生化国家开放重点实验室 [2]Department of Medicine,Mount Sinai School of Medicine New York,NY10029

出　　处：《云南植物研究》2006年第4期425-428,共4页Acta Botanica Yunnanica

基　　金：国家自然科学基金资助(39730280)

摘　　要：微管(microtubule)是细胞骨架的重要成份,参与囊泡运输、信息传递等多种生命活动。我们从萱草花粉中纯化了植物微管蛋白,对其生物化学及生物物理学部分性质研究表明,纯化的微管蛋白经超离心法测定沉降系数为6.2S,SDS-PAGE分析α,β微管蛋白分子量为56kD、58kD,凝胶扫描分析纯度为93.7%。等电聚焦电泳测定等电点为pI=5.35。光谱学性质研究结果表明,最大紫外吸收峰为280.8nm,荧光光谱研究表明最大激发波长为282nm;此时的最大发射峰为338nm,圆二色光谱分析二级结构表明α-螺旋占27.24%,β-折叠占24.48%,无规卷曲为48.28%,呈典型球蛋白特征。Euearyotie cells contain a complex network of filamentous proteins collectively called the eytoskeleton, which participates in many cellular functions, including organelle motility and information transfer. Tubulins are one of the main components of the cell eytoskeleton. Tubulins of high purity were prepared from day lily pollen grains, and were biochemically and biophysically characterized in this study. The molecular weight of α-and β-tubulin from day lily pollen is about 56 kD and 58 kD on SDS-PAGE, respectively. The purity is 93.7% by scanning analysis. The tubulin has a sedimentation coefficient of 6.2S and an isoelectrie point of about 5.35. The maximum ultraviolet absorption is 280.8 nm. Fluorescence emission wave length of day lily tubulin is 338 nm by excitation at 282 nm. Circular diehroism （CD） spectrum analysis showed that the percentage of α-helix, β-sheet and random coil of day lily tubulin is 27.24%, 24.48% and 48.28%, respectively, indicating a typical feature of globulin.

关 键 词：微管蛋白 微管 花粉 萱草 

分 类 号：Q946[生物学—植物学]