抗SARS冠状病毒N蛋白多克隆抗体的制备和鉴定  

Preparation and characterization of polyclonal antibody against N protein of SARS-CoV

作　　者：戚扬[1] 文翠容[1] 迟淑萍[1] 孙杰[1] 朱雷[1] 周继文[1] 程云[1] 

机构地区：[1]解放军第302医院传染病研究所免疫室,北京100039

出　　处：《解放军医学杂志》2006年第8期781-783,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助项目(30340010);北京市自然科学基金资助项目(7034050)

摘　　要：目的制备抗SARS冠状病毒(SARS-CoV)N蛋白的多克隆抗体。方法以纯化的GST-N免疫新西兰白兔,制备抗SARS-CoVN蛋白多抗,间接ELISA法检测兔血清效价,Westernblot和免疫组化鉴定多抗的特异性。结果制备的抗SARS-CoVN蛋白多克隆抗体经ELISA检测效价为1·2×10-5,Westernblot和免疫组化证实此抗体可与SARS-CoVN蛋白特异性结合。结论成功地制备了抗SARS-CoVN蛋白多抗,为进一步的临床诊断和实验研究创造了条件。Objective To prepare and characterize the polyclonal antibody against N protein of SARS-CoV. Methods The polyclonal antibody against SARS-CoV N was obtained from immunized rabbit with purified GST-N. The titer of the antibody was determined by indirect ELISA, and the specificity by Western blot and imrnunochemical staining. Results The rabbit＇s antibody against SARS-CoV N was prepared successfully. The titer of antiserum against SARS-CoV N was about 1.2 × 10^-5. Western blot and imrnunochemical staining analysis showed that the polyclonal antibody could bind to the expressed fusion protein specifically. Conclusion The rabbit＇s antibody against SARS-CoV N has been prepared successfully, and it can be a useful reagent for clinical diagnosis and further research.

关 键 词：SARS病毒 N蛋白 抗体 病毒 

分 类 号：R392.116[医药卫生—免疫学]