黄山短尾猴mtDNA控制区序列变异及种群的遗传多样性  

Sequence variation of mitochondrial DNA control region and population genetic diversity of Tibetan macaques Macaca thibetana in the Huangshan Mountain

作　　者：柳杨[1] 李进华[1] 赵健元[1] 

机构地区：[1]安徽大学生命科学学院

出　　处：《动物学报》2006年第4期724-730,共7页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(No.39970109;No.30570237);教育部骨干教师资助计划(No.18557);安徽省优秀青年基金(No.04043070);安徽省教育厅自然科学基金(No.2005KJ021ZD);安徽大学学术创新团队项目资助~~

摘　　要：短尾猴属灵长目(Primates)猴科(Cercopithecidae)猕猴属(Macaca),是我国特有的国家二级保护动物。为了更有效地保护其野生种群,本文研究了黄山短尾猴种群内的遗传多样性,并对黄山短尾猴与四川短尾猴种群间的遗传差异进行了分析。共测定了黄山短尾猴7个群体中的30个样本的mtDNA控制区5′端493bp的序列,只发现了7个变异位点,定义了3种单倍型,单倍型序列之间缺乏变异,种群中的核苷酸多样性很低(0.006);3种单倍型相应地将黄山种群分为了3个亚群,不同亚群之间呈现出一定的片断化分布,从分子水平上初步揭示了短尾猴黄山种群的遗传多样性。与四川短尾猴的相应序列比较,黄山短尾猴控制区序列存在很大差异,共有59个变异位点,而且存在大片段的碱基插入/缺失,有78%的遗传变异发生在两个种群之间,两个种群间的核苷酸歧异度已达8.21%。进一步分析表明,黄山短尾猴与四川短尾猴之间存在着极显著的遗传分化(FST=0.399,P<0.001),基于最大似然法和邻接法构建的系统发生树均将两者聚为不同的类群,支持将它们归入各自的管理单元。Tibetan macaque Macaca thibetana is endemic to China and is listed as a category Ⅱ species of the state key protected wildlife. To study the genetic diversity of the Tibetan macaque population in the Huangshan Mountain and its genetic variation with the population in Sichuan Province, we sequenced the left domains of the mtDNA control region of 30 samples, which were obtained from 7 Tibetan macaque groups dispersed in the Huangshan Mountain. Only 7 variable sites were detected in the nucleotide sequences of 493 bp, all of which were the transitions between T and C. Three haplotypes were identified, and according to the distribution of the haplotypes, we can generally divide the Huangshan population into three subpopulations, We didn＇ t detect a haplotype shared between each two subpopulations and the genetic diversity of the whole Huangshan population was only 0. 006. The sequences we obtained varied considerably with the corresponding ones of the Sichuan Tibetan macaques, with 59 mutant sites and large insert/deletion. The genetic variation and the nucleotide divergence between the Huangshan population and the Sichuan population were 78 % and 8.21,%. The phylogenetic tree and AMOVA analysis demonstrated that there were distinctive genetic divergences between the two Tibetan macaque populations （FST= 0. 399, P 〈 0. 001 ）, supporting the treatment of the Huangshan and Sichuan Tibetan macaque populations as two different management units [Acta Zoologica Sinica 52 （4）： 724- 730, 2006].

关 键 词：短尾猴 MTDNA控制区 遗传多样性 序列变异 

分 类 号：Q953[生物学—动物学]