金黄色葡萄球菌对心脏瓣膜巨噬细胞集落刺激因子及其受体基因表达影响的实验研究  

Cardiac valve MCSF-1 and its receptor expression changes in Staphylococcus aureus induced infective endocarditis

作　　者：赵波[1] 吴亮[2] 孙威[1] 付向宁[1] 李军[1] 潘铁成[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胸心外科,武汉430030 [2]华中科技大学同济医学院附属同济医院感染科,武汉430030

出　　处：《中华心血管病杂志》2006年第8期744-746,共3页Chinese Journal of Cardiology

基　　金：同济医院科研基金资助项目(2096699486)

摘　　要：目的初步探讨金黄色葡萄球菌对心脏瓣膜巨噬细胞集落刺激因子(MCSF-1)及其受体的影响和感染性心内膜炎(IE)的发病机制。方法高低两种剂量金黄色葡萄球菌注射联合手术造成心脏瓣膜损伤的方法分别建立4组IE家兔模型;RT-PCR法检测各组瓣膜的MCSF-1及其受体(c-fms)mRNA的表达水平。结果32例动物中存活26例,其中14/26(53.8%)例发生IE。小剂量组中赘生物产生、细菌培养阳性率显著低于大剂量组(P<0.05)。手术+细菌注射可诱导二尖瓣、三尖瓣MCSF-1 mRNA表达明显增加,c-fms mRNA表达明显降低。大剂量细菌注射比小剂量细菌注射诱导二尖瓣MCSF-1 mRNA表达更明显(P<0.01),三尖瓣表达差异无统计学意义。结论MCSF-1/ c-fms可能是参与IE发生的重要因子之一。左、右心腔对大剂量细菌入侵有不同的抗感染免疫反应,这可能是左、右心IE有不同临床特点的原因之一。Objective To explore the cellular immunology mechanism of infective endocarditis （IE）, we investigated the effects of Staphylococcus aureus （S. aureus ） on MCSF-1 and its receptor （c-fms） gene expression in cardiac valves. Methods Thirty-two rabbits were divided into 4 groups： mitral or tricuspid valve artificial lesions with 5 × 10^4 CFU or 5 × 10^6 CFU S. aureus injection. Control rabbits （ n = 7） received 5 × 10^6CFU S. aureus injection. IE after operation were confirmed by naked eyes and electron microscope observations. MCSF-1, c-fms in mitral and tricuspid valves were detected by RT-PCR. Results Twenty-six rabbits survived the operation and 14 rabbits developed IE （2 with 5 × 10^4 CFU and 12 with 5 × 10^6 CFU S. aureus injection ） one day post operation. S. aureus injection alone did not induce IE. Compared to control rabbits, MCSF-1 mRNA was significantly upregnlated and c-fms mRNA significantly downregnhed after 5 × 10^4 CFU S. aureus injection with heart valve artificial lesion in mitral valves or tricuspid valves. MCSF-1 expression in mitral valves was further increased while remained unchanged in tricuspid valve after 5 × 10^6 CFU S. aureus injection compared to that in 5 × 10^4 CFU S. aureus injection group. Conclusion High dose bacterial invasion and heart valves lesion were the main factors for inducing infective endocarditis. Development of infective endocarditis was associated with valve MCSF-1/c-fms expression changes in this rabbit model.

关 键 词：心内膜炎 细菌性 葡萄球菌 金黄色 巨噬细胞集落刺激因子 基因 c-fms 

分 类 号：R542.4[医药卫生—心血管疾病]