幼年特发性关节炎患儿外周血Th细胞亚群变化  

T-help lymphocyte and its subsets in peripheral blood of children with juvenile idiopathic arthritis

作　　者：卢燕鸣[1] 曹兰芳[1] 马敏[1] 薛海燕[1] 赵瑜[1] 余汉卿[1] 毛海英[1] 顾越英[1] 

机构地区：[1]上海交通大学医学院附属仁济医院儿科风湿病GROPEC组,上海200001

出　　处：《临床儿科杂志》2006年第8期666-669,共4页Journal of Clinical Pediatrics

摘　　要：目的探讨幼年特发性关节炎(JIA)患儿外周血CD4+T淋巴细胞及其亚群CD4+CD45RA+、CD4+CD45RO+的表达及其临床意义。方法采用免疫荧光标记技术和流式细胞仪检测36例JIA患儿外周血CD4+T淋巴细胞及其亚群CD4+CD45RA+、CD4+CD45RO+的表达,同期检测20例年龄、性别无差异的健康儿童为对照。结果JIA患儿外周血CD4+T淋巴细胞明显低于对照组(t=2.099,P<0.05),CD4+CD45RA+T淋巴细胞数与对照组比较明显降低(t=3.450,P<0.01),CD4+CD45RO+T淋巴细胞数明显升高(t=3.913,P<0.01),CD4+CD45RA+T/CD4+CD45RO+T比值明显降低(t=4.904,P<0.01);与对照组比较,JIA各亚型(全身型、多关节型、少关节型)的CD4+CD45RO+T淋巴细胞数均明显升高,CD4+CD45RA+T/CD4+CD45RO+T比值明显降低(P<0.01);CD4+CD45RA+T淋巴细胞数与正常对照组比较在全身型中显著降低(t=4.192,P<0.01);在多关节型中明显降低(t=2.214,P<0.05);在少关节型中稍有降低,但与对照组比较差异无显著性(t=1.793,P>0.05)。结论JIA患儿的免疫功能紊乱主要表现为CD4+T淋巴细胞及其亚群CD4+CD45RA+T/CD4+CD45RO+T失衡,这可能在JIA发病机制中起着重要的作用。Objective To explore the expression of CD4^＋ T （T-help lymphocyte） and its subsets CD4^＋CD45RA^＋, CD4^＋CD45RO^＋ in peripheral blood （PB） of children with juvenile idiopathic arthritis （JIA）. Methods The expression of CD4^＋T and CD4^＋CD45RA^＋,CD4^＋CD45RO^＋ in PB were measured using flow cytometry and monoclonal antibodies in 36 ehildren with JIA. Twenty healthy children matched in age and gender served as normal controls. Results The CD4^＋ T was significantly lower in children with JIA than that in normal controls （t = 2.099,P 〈 0.05）. The percentage of CD4^＋CD45RO^＋T （t = 3.913,P 〈 0.01 ） significantly increased whereas the percentage of CD4^＋CD45RA^＋T （t = 3.450,P 〈 0.01 ） deceased in children with JIA,and there was an obvious decrease in the ratio of CD4^＋CD45RA^＋T/CD4^＋CD45RO^＋T in comparison with those in normal controls （P 〈 0.01）. Furthermore,the increased percentage of CD4^＋CD45RO^＋T and the decreased ratio of CD4^＋CD45RA^＋T/CD4^＋CD45RO^＋T were observed in all three types of JIA （systemic arthritis,polyarticular arthritis and oligoarthritis） in comparison with those in normal controls （P 〈 0.01 for all）. The percentage of CD4^＋ CD45RA^＋T decreased significantly in children with either systemic arthritis or polyarticular arthritis in comparison with that in normal controls （t = 4.192,P 〈 0.01 ;t = 2.214,P 〈 0.05）. There was no statistical difference between children with oligoarthritis and normal controls in the percentage of CD4^＋CD45RA^＋T （t = 1.793,P 〉 0.05）. Conclusions The disturbance of immune function appeared in children with JIA mainly presents as dysfunction of CD4^＋T and imbalance of CD4^＋ CD45RA^＋T and CD4^＋CD45RO^＋T,which may play an important role in the pathogenesis of JIA probably.

关 键 词：幼年特发性关节炎 Th淋巴细胞 CD4^+CD45RA CD4^+CD45RO 儿童 

分 类 号：R725.9[医药卫生—儿科]