粳稻成熟胚高频率再生因素的研究  

Factors Affecting the Frequencies of Callus Induction and Plantlet Regeneration in Mature Embryo Culture of Rice

作　　者：郝文媛[1] 王丕武[2] 林秀峰[1] 姚丹[2] 武丽敏[2] 

机构地区：[1]吉林省农业科学院,吉林公主岭136100 [2]吉林农业大学生物中心,长春130118

出　　处：《吉林农业科学》2006年第4期30-33,共4页Journal of　Jilin Agricultural Sciences

基　　金："国家转基因植物中试及产业化基地"专项基金(J99-B-001);吉林省科技厅项目资助

摘　　要：以水稻成熟胚为外植体,就不同基因型、不同激素浓度对愈伤组织诱导、继代及分化的影响进行研究,并建立了水稻成熟胚高频再生系统。研究结果表明:NMB、NB、CC为较适宜的培养基,可以用于北方粳稻愈伤组织的诱导;在诱导过程中加入2,4-D1～2mg/L有利于愈伤组织的诱导;在继代的过程中加入甘露醇4g/L可明显改善愈伤组织的质量;诱导分化时,进行预分化-分化培养可以显著提高分化率。Five rice genotypes were used to study the factors affecting callus induction and subsequent plantlet regeneration in mature embryo culture of rice. Highly efficient plantlet regeneration systems were established for tested varieties. The results were as follows： （1） Medium of NMB, NB and CC gained very well induction efficiency and excess 50 percent. （2） Induction medium which containing 2,4-D 1-2mg/L can obviously enhance the efficiency of induction. （3） D-Mannital 4g/L can improve the state and height of calli. （4） Treat- ment of calli for pre-regeneration before differentiation could obviously increase plantlet regeneration.

关 键 词：水稻 成熟胚 高效 再生系统 

分 类 号：S511.035.3[农业科学—作物学]