天癸更年软胶囊对老年雌性大鼠骨质疏松作用的实验研究  

Experimental Study on the Effect of Tiangui Gengnian Capsule on the Aged Female Rats Ostepoprosis

作　　者：刘波[1] 袁秉祥[1] 郭雄[2] 魏霞蓁 赵丽梅[1] 陈东 康军 

机构地区：[1]西安交通大学医学院药理学系,陕西西安710061 [2]教育部地方病骨病研究所,陕西西安710061 [3]西安交大天奎医药生物公司,陕西西安710061

出　　处：《中药材》2006年第8期803-806,共4页Journal of Chinese Medicinal Materials

基　　金：国家863专项基金(No.2004AA2Z3980)

摘　　要：目的:观察天癸更年软胶囊所含内容物沙棘脂肪酸对老年雌性大鼠骨质疏松的治疗作用,并从激素及细胞因子方面探讨其抗骨质疏松的作用机理。方法:分别测定青年雌性大鼠、22月龄老年雌性大鼠及沙棘脂肪酸(0.72 g/kg、1.8 g/kg、4.5 g/kg)和己烯雌酚(0.02 g/kg)灌胃给药45天后的22月龄老年雌性大鼠的股骨及椎骨的骨密度(BMD)、骨生物力学、骨形态学变化以及血清雌激素(E2)、转化生长因子TGF-β1和胰岛素生长因子IGF-1水平。结果:老年雌性大鼠骨密度(BMD)、生物力学、形态学水平均发生变化,与青年组比较有统计学意义(P<0.05),证明了老年鼠存在程度不等的骨质疏松。与老年空白组比较,沙棘脂肪酸实验治疗组(1.8 g/kg、4.5 g/kg)大鼠骨密度、生物力学、骨小梁数目及皮质厚度上升(P<0.05),血清TGF-β1和IGF-1水平升高(P<0.05)。结论:沙棘脂肪酸能有效改善老年大鼠的骨骼代谢,减缓并纠正骨质疏松的发病,其作用机制可能是通过影响激素及细胞因子来实现的。Objective： To study the therapeutic action of Seabuckthorn Fatty Acid on the aged female rats osteoporosis, and explore the corresponding mechanism related in hormone and eytokine. Methods： The change of bone density（ BMD）, biomeehanies and bone morphology were measured and E2 , TGF-β1, IGF-1 level in plasma were determined respectively for the young female rats, aged female rats（ Seabuekthorn Fatty Acid 0. 72,1.8,4. 5 g/kg, E2 0.02 g/kg） after they were administrated corresponding drugs for 45 days. Results： The bone density （BMD）, bone biomeehanies, bone morphology for female rats aged 22-months all decreased. The BMD, bone biomeehanies, the number of bone trabeeulas and cortical thickness in Seabuekthorn Fatty Acid treated groups（ 1.8 g/kg, 4.5 g/kg） were upgraded（P 〈 0. 05）. The TGF-β1, IGF-1 level in plasma were increased, too（P 〈 0. 05）. Conclusion ： Seabuekthorn Fatty Acid can meliorate bone metabolism in post-menopause and heighten the bone density. The adjustment mechanism may be based on the function of estrogen and cell eytokine.

关 键 词：绝经后骨质疏松 天癸更年软胶囊 沙棘脂肪酸 骨形态学 生物力学 

分 类 号：R285.5[医药卫生—中药学]