豆天蛾核型多角体病毒F蛋白基因的克隆和序列分析  

Cloning and Sequence Analysis of F Protein Gene of Clanis bilineata Nuclear Polyhedrosis Virus

作　　者：庞俊星[1] 易建平 朱姗颖[1] 王文兵[1] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013 [2]上海市出入境检验检疫局,上海200135

出　　处：《安徽农业科学》2006年第15期3621-3624,共4页Journal of Anhui Agricultural Sciences

基　　金：江苏省六大人才高峰基金项目(2005-农-21);江苏大学自然科学创新预研基金项目(04CX08)

摘　　要：从豆天蛾幼虫虫体中分离纯化出一种新型的豆天蛾核型多角体病毒（CbNPV），提取基因组DNA，进行基因组测序。结果发现一个与杆状病毒F蛋白基因同源性很高的读码框序列，该长度为2109bp，编码702个氨基酸。氨基酸序列同源性分析表明：CbNPVF蛋白与Ⅱ类NPV和Ⅰ类NPVF蛋白的同源性分别为32．9％～61．8％和8．0％～16．1％，并且将它与多种Ⅰ类NPV杆状病毒同功能蛋白GP64进行分析，同源性在9．1％～11．4％，表明CbNPVF蛋白与Ⅰ类NPV的F及GP64蛋白的同源性很低，因此认为CbNPV属于Ⅱ类NPV。Clanis bilineata Nuclear Polyhedrosis Virus（CbNPV）was purified from Clanis bilineata larva. To obtain the molecular information of the vires, the genomie DNA of CbNPV was extracted, and a DNA fragment library of the vires was constructed by Shotgun. The positive clones were sequenced and analyzed. A new open reading frame, which had high identifieatiou with the F protein gene of most baeulovimses, was found in the library. The F protein gene of CbNPV was 2 109 bp long and encoded a protein with 702 amino acids. The characteristic and structure of the sequence was .analyzed. The amino acid sequence analysis indicated that the CbNPV F protein had 8.0% - 16.1% and 32.9% - 61.8% identities with Group Ⅰand Ⅱ NPVs F proteins, respeetively, And then to be compared with many kinds of group ⅠNPVs gp64 gene, the result indicated that the）, had 9. 1% - 11,4% homology, which showed that CbNPV belolonged to Group Ⅱ NPV.

关 键 词：豆天蛾核型多角体病毒 F蛋白 序列 分析 gp64基因 

分 类 号：Q946[生物学—植物学]