福辛普利预处理对缺氧复氧心室肌L型钙通道电流的影响  

Effect on L type calcium currents of fosinopril late preconditioning in ventricular myocytes during anoxia/reoxygenation period

作　　者：田志广[1] 张文杰[2] 郑杨[3] 

机构地区：[1]温州医学院附属第一医院心内科,浙江温州325000 [2]吉林大学基础医学院生理教研室 [3]吉林大学第一医院心内科,130021

出　　处：《心血管康复医学杂志》2006年第4期368-371,共4页Chinese Journal of Cardiovascular Rehabilitation Medicine

摘　　要：目的:观察福辛普利晚期预处理对缺氧复氧心室肌细胞L型钙通道电流的影响。方法:应用全细胞膜片钳方法,记录酶解的成年豚鼠心室肌细胞及福辛普利预处理后L型钙通道电流在缺氧复氧期的变化。结果:缺氧复氧期心室肌细胞L钙电流峰值明显下降,与对照组相比有明显差异(-237.71±17.70pAvs-286.00±21.15pA,P=0.0006,n=7),L型钙电流电流-电压曲线上移;福辛普利预处理后心室肌细胞L钙电流峰值亦下降,但下降不明显(-265.57±20.20PAvs-286.00±21.15pA,P=0.089,n=7)与缺氧复氧组比较有明显差异(P=0.018),L型钙电流电流-电压曲线上移幅度减小。结论:福辛普利可稳定缺氧复氧心肌L型钙通道电流,对心肌有保护作用。Objective： To investigate effect on L type calcium currents of fosinopril late preconditioning in ventricular myocytes during anoxia/reoxygenation period. Methods： Using whole-patch clamp cinefiguration in isolated adult guinea pig ventricular myocytes with an enzymatic procedure the L-type calcium current were measured before and after ischemic application of each group respectively. Results： Under the condition of reoxygenation, the peak of ICa-L reduced significantly（-237.71±17.70 pA vs. -286.00±21.15 pA,P=0. 0006,n= 7） and ICa-L Ⅰ～Ⅴ curve shifted to upper. After fosinopril preconditioning, the peak of ICa-L insignificantly reduced compared to control （-265.57± 20. 20 PA vs. ±286.00± 21.15 pA, P= 0. 089, n= 7）, but compared with reperfusion group its difference to reperfusion group was significant （P=0. 018）, its ICa-L Ⅰ～Ⅴ curve shifted to under. Conclusion： Fosinopril can stabilize L type calcium currents in myocytes during anoxia/reoxygenation, so possess the protective effect for ventricular myocytes.

关 键 词：晚期预处理 膜片钳术 钙通道 L-型 

分 类 号：R542.2[医药卫生—心血管疾病]