高效液相色谱法测定甲氧苄啶氯化钠注射液中甲氧苄啶的含量  

Quantification of Trimethoprim in Trimethoprim and Sodium Chloride Injection Solution by HPLC

作　　者：谢燕[1] 陈明[2] 肖艳萍[1] 

机构地区：[1]湖南医药工业研究所,湖南长沙410014 [2]湖南化工研究院测试中心,湖南长沙410007

出　　处：《精细化工中间体》2006年第4期64-66,共3页Fine Chemical Intermediates

摘　　要：建立了甲氧苄啶氯化钠注射液中甲氧苄啶含量的HPLC测定方法。本法采用C18色谱柱,流动相为0.1mol/L磷酸二氢钾溶液(用磷酸调节pH值至2.5)-甲醇的混合溶液(按v∶v=70∶30配制),检测波长为280nm,流速为1.0mL/min。实验数据表明:甲氧苄啶在0.38～1.37μg的范围内,峰面积与进样量呈良好线性关系(r=0.9995),回收率RSD=0.69%,最低检测限为1ng,分析结果准确,可用于测定甲氧苄啶氯化钠注射液中甲氧苄啶含量。A HPLC method for trimethopfim in trimethoprim and sodium chloride injection solution was establish. The HPLC condition consisted of C18 column （250 mm×4.6 mm, 5μm） , mobile phase of 0.1 mol/L monopotassium phosphate-methanol （70 ： 30, v/v） at the flow rate of 1.0 mL/min and at detection wavelength 280 nm. The calibrattion linear curve of trimethoprim was in the range of 0.38-1.37μg （r=0.999 5） with RSD of 0.69%, and the detection limit was 1 ng. This method was very sensitive and reliable, and could be used to measure trimethopfim in trimethoprim and sodium chloride injection solution.

关 键 词：甲氧苄啶氯化钠注射液 高效液相色谱法 甲氧苄啶 

分 类 号：O621.3[理学—有机化学]