ADD-1基因和GNB3基因多态性与原发性高血压的相关性研究  

Association analysis between genetic polymorphism of ADD-1 gene and GNB3 gene and essential hypertension

作　　者：董会奕[1] 李秋荣[1] 王勤[1] 罗振国[2] 

机构地区：[1]北京大学深圳医院心内科,深圳518036 [2]深圳大学生命科学学院,深圳518060

出　　处：《岭南心血管病杂志》2006年第4期258-261,共4页South China Journal of Cardiovascular Diseases

摘　　要：目的调查ADD-1基因和GNB3基因多态性与深圳地区原发性高血压的关系。方法用病例对照研究。高血压组97例,非高血压组87例。用MS-PCR和PCR-RFLP方法分别检测ADD-1基因G460T基因型及GNB3基因T825C基因型。结果①高血压组和非高血压组ADD-1基因型分别为GG0.237/0.241、GT0.505/0.460、TT0.258/0.299,差异无统计学意义(P=0.787);G等位基因频率为0.490/0.471,差异无统计学意义(P=0.724);②高血压组和非高血压组GNB3基因型分别为TT0.258/0.161、TC0.484/0.529、CC0.258/0.310,差异无统计学意义(P=0.265);T等位基因频率为0.5/0.425,差异无统计学意义(P=0.151);③联合基因分析高血压组TT+CC联合基因型者显著少于非高血压组(P=0.043)。结论在深圳地区人群中,未发现ADD-1基因多态及GNB3基因多态与高血压相关,但两基因可能存在协同作用。Objiectives To investigate the association between the polymorphism of ADD-1 gene and GNB3 gene and essential hypertension （EH） in Shenzhen. Methods The case-control study was conducted in 97 cases with EH（EH group） and 87 normal controls（NC group）. The genotypes of ADD-I gene was detected by MS-PCR and that of GNB3 gene by PCR-RFLP. Results （1）There were no significantly different in genotype distribution of ADD-1 gene （GG 0.237 vs 0.241,GT 0.505 vs 0.460,IT 0.258 vs 0.299,P=0.787） and GNB3 gene （IT 0.258 vs 0.161,TC 0.484 vs 0.529,CC 0.258 vs 0.310, P=0.265）between EH group and NC group; （2）There were no significantly different in G allele frequency of ADD-1 （0.490 vs 0.471,P=0.724） and T allele frequency of GNB3 gene （0.5 vs 0.425,P=0.151） between two groups; （3）The genotype distribution of IT＋CC of the EH group （5.15 per cent）was significantly lower than the NC group （12.64 per cent,P=0.043）. Conclusions These observations suggested that ADD1 G460T polymorphism and GNB3 C825T polymorphism are not associated with essential hypertension in Shenzhen population, but there might be a coordinating effect between the T allele of ADD1 and the C allele of GNB3.

关 键 词：原发性高血压 ADD-1基因 GNB3基因 基因多态性 

分 类 号：R544.1[医药卫生—心血管疾病]