H1N1亚型猪流感病毒血凝素基因的克隆与表达  

Cloning and Expressiong of Subtype H1N1 SIV HA Gene in Vero Cells

作　　者：马祥[1] 顾万军[2] 刘镇明[1] 黄良宗[2] 彭启明[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]佛山科学技术学院,广东佛山528231

出　　处：《动物医学进展》2006年第8期73-76,共4页Progress In Veterinary Medicine

基　　金：广东省自然科学基金资助项目(34064)

摘　　要：应用RT-PCR方法扩增了1株H1N1亚型猪流感病毒的血凝素基因,并克隆到pMD 18-T Simple载体中,经PCR、酶切和测序验证克隆正确后,亚克隆到真核表达载体pcDNA3.1(+)中,构建了重组质粒pcDNA-HA。在脂质体作用下重组质粒pcDNA-HA转染Vero细胞。间接免疫荧光试验结果表明,血凝素基因在Vero细胞中成功进行了瞬时表达,这为猪流感病毒DNA疫苗的进一步研究奠定了基础。The HA gene of subtype HIN1 SIV was amplified by RT-PCR assay and sequently subcloned into vector pcDNA3. 1 （＋） to construct expression plasmid pcDNA-HA. Mediated with Lipofectamine TM 2000,pcDNA-HA was introduced into Veto ceils. And the indirect immuno fluorescent assay analysis showed that HA gene was been expressed transiently in Veto cells.

关 键 词：猪流感病毒 血凝素基因 克隆 表 达 VERO细胞 

分 类 号：S852.659.5[农业科学—基础兽医学]