对虾白斑综合症病毒(WSSV)结构蛋白VP28与VP26的功能分析  

Functional Analysis on Structure Proteins VP28 and VP26 of White Spot Syndrome Virus

作　　者：刘非[1] 寸树健[1] 杨凯[1] 邓百煌[1] 杨晓仪[1] 吴文言[1] 

机构地区：[1]中山大学有害生物控制与资源利用国家重点实验室//生物医药中心,广东广州510275

出　　处：《中山大学学报（自然科学版）》2006年第4期83-86,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家重点基础研究发展规划资助项目(G1999012002);国家自然科学基金资助项目(39970583);广东省自然科学基金资助项目(001216);广东省重点攻关资助项目(A3050303)

摘　　要：对虾白斑综合症病毒(W SSV)结构蛋白功能的研究数据还不多。怎样预测并验证那些新发现的蛋白功能,为实验研究提供理论参考,是功能基因及结构蛋白的研究重点之一。利用生物信息学技术对获得的W SSV相关数据进行分析和归纳。分析数据显示结构蛋白VP28与VP26在二级结构和跨膜结构域等方面具有很高的相似度;而且,这两个蛋白与任何已知的蛋白序列没有明显的同源性,但二者的氨基酸序列彼此间具45%的相似性,提示这两个蛋白可能执行相似的生理功能。一个特别设计的细胞吸附实验用于进一步研究W SSV侵染宿主细胞的过程中VP28和VP26所扮演的角色。实验结果显示,两个蛋白均可以与对虾鳃组织来源的细胞发生特异的体外吸附,表明VP28和VP26可能参与W SSV侵染宿主细胞的初始阶段。Research about white spot syndrome virus （WSSV） structure proteins is not enough. Bio-information analysis shows that the structure proteins, VP28 and VP26, have 45% similarity of amino acid sequence, besides their high similarity in secondary structure and trans-membrane domains, which implies that the two proteins might evolve through gene duplication. It could be supposed that VP28 and VP26 proteins might execute similar function, since considering animal DNA viruses there are rarely proteins originated from a common ancestral gene have functionally diverged. A novel cell-binding experiment is designed to study what role VP28 and VP26 proteins play in the process of WSSV infecting host cells. The results show that the two proteins can bind shrimp ceils specifically, whereas the control protein GFP could not. Possibly, the two proteins are involved in the initiation stage of infectious procedure, that is to say they may perform similar function. The results support our hypothesis presented according to the outcome of bioinformatics analysis.

关 键 词：WSSV VP28 VP26 生物信息学 细胞吸附 

分 类 号：Q51[生物学—生物化学] S945.46[农业科学—水产养殖]