用ISSR分子标记检测不同尾矿废弃地白茅居群的遗传多样性  

Genetic Diversity of Imperata cylindrica Populations Colonizing on the Metal Tailings Waste Lands by ISSR Technique

作　　者：田胜尼[1] 王峥峰[1] 高三红[2] 郝艳茹[1] 彭少麟[1] 

机构地区：[1]中国科学院华南植物园∥广东省数字植物园重点实验室 [2]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2006年第4期87-92,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(30270282);广东省优秀团队资助项目(003031);广东省重大基金资助项目(B20801002)

摘　　要：采用ISSR技术对安徽4个铜尾矿、1个铅锌尾矿废弃地上自然定居的白茅和非矿区正常生境下1个白茅居群遗传多样性进行了研究。从100条ISSR引物中筛选出10条引物对6个居群180个样品进行PCR扩增,共得到139扩增带,其中多态位点为132条,多态位点百分率为94.96%。POPGENE分析表明,白茅居群的总遗传多样性为0.3125,非矿区白茅的遗传多样性远高于另外5个矿区居群,铅锌尾矿的遗传多样性最低,6个白茅居群之间出现明显的遗传分化(φst=0.030,P<0.002),各居群的个体各自聚在一起。上述结果可能是在金属尾矿废弃地胁迫下,白茅居群遗传本质上产生了明显的遗传分化。The genetic diversities of six I. cylindrica populations were determined by ISSR technique . The sample materials were collected in Anhui province from four different copper railings, one lead - zinc tailings waste land and the normal soil far away from mine areas. Out of 100 ISSR primers, 10 primers generated highly reproducible and stable DNA bands. Using these primers, 139 discernible DNA fragments were produced for the 180 samples of six I. cylindrica populations. The number of polymorphic loci was 132, and the percentage of polymorphic loci was 94.96%. The result of POPGENE analysis indicated the whole genetic diversity （Ht） of I. cylindrica was 0. 312 5, and the genetic diversity （Hs） among the populations was 0. 226 5. The genetic diversity, Shannon index and percentage of polymorphic loci for the population growing on the normal soil were higher than other five populations on the metal tailings waste land. The results of AMOVA and PCA analysis indicated genetic differentia- tion had taken place signally among the six populations colonizing （ ψst = 0. 03.0, P 〈 0. 002）. From the results of this studies, the high genetic diversity of I. cylindrical may contribute to colonize on the copper tailings and lead - zinc tailings.

关 键 词：ISSR 金属尾矿废弃地 白茅 遗传多样性 

分 类 号：Q16[生物学—普通生物学]