水样预处理法分离溶藻细菌效果观察  

An efficient method screening algicidal bacteria from fresh water

作　　者：李永欣[1] 韩绍印[1] 席宇[1] 吴刚[2] 赵以军[2] 

机构地区：[1]郑州大学生物工程系,郑州450052 [2]华中师范大学生命科学学院,武汉430079

出　　处：《郑州大学学报（医学版）》2006年第5期865-867,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：留学回国人员科研启动基金资助项目教外司[2000]367号;武汉市科技攻关计划基金资助项目20026002097;"211"工程肿瘤与生物工程基金资助项目教重办2002-2号

摘　　要：目的:探讨如何从水华期采集的水样中有效地分离溶解蓝藻的细菌。方法:从富营养化池塘中采集表层水样,采集的水样分3等份,分别进行如下处理:①经0.8μm纤维滤膜过滤处理。②0.22μm纤维滤膜过滤,氯仿剧烈震荡处理5min。③103.42kPa、121.5℃条件下灭菌20min。将3种处理水样分别与液体培养供试蓝藻共培养,通过溶藻试验、富集培养和平板划线法筛选溶藻细菌。结果:筛选出7株能够抑制供试蓝藻生长或者溶解供试蓝藻的细菌。结论:本方法能有效地分离溶藻细菌,值得推广。Aim： To explore an efficient isolation method of algicidal bacteria. Methods：Surface water samples were collected from several eutrophic ponds, and water samples were divided into three parts： one aliquot of water samples were filtered through a 0. 8μm pore size nuclepore filter; the second aliquots were first filtered through a 0. 22μm pore size nuclepore filter, then treated violently by chloroform for 5 min; the last aliquots were autoclaved at 103.42 kPa and 121.5 ℃ for 20 min. With the three treated water samples, algicidal tests, enriched culture and streak plate method were conducted. Results： By the above technique, 7 strains of algicidal bacteria were obtained. Conclusion： This modified screening method is effeicient for isolation of algicidal bacteria and is worth popularizing.

关 键 词：有害藻类 水华 溶藻细菌 分离 

分 类 号：Q949.220.5[生物学—植物学]