大肠杆菌甜菜碱醛脱氢酶基因的克隆及其在烟草中的表达  

Cloning of the Betaine Aldehyde Dehydrogenase Gene from Escherichia coli and its Expressions in Tobacco

作　　者：陆云华[1] 马立新[1] 张新[2] 

机构地区：[1]湖北大学生命科学学院分子微生物与基因工程实验室,湖北武汉430062 [2]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《安徽农业科学》2006年第14期3308-3309,共2页Journal of Anhui Agricultural Sciences

基　　金：国家"863"项目(2002AA227011);湖北省自然科学基金资助项目(2003ABA118)

摘　　要：利用PCR克隆了大肠杆菌甜菜碱醛脱氢酶基因(betB),构建了含betB基因的重组植物表达质粒载体pLM47,并用农杆菌介导的叶圆盘法对烟草叶片进行遗传转化。结果表明,在含有潮霉素的MS筛选分化培养基上筛选,获得基因转化烟草植株3株;经报告基因GVS的组织化学检测和Western blot检测,证明betB基因在烟草转化植株中得到了表达。The betaine aldehyde dehydrogenase gene-betB from Escherichia coli XL1 -gold was cloned by PCR and the gene was inserted into a binary vector to get the pLM47 vector. The pLM47 vector was transformed into agrobacteriun tumefaciens （LBA4404）and then LBA4404 was transformed into tobacco（Nicotiana tobacum） plant with leaf disc transformation, After grown on the screening medium containing hygromycin, 3 transformants were obtained. The gene-betB in transformants was confirmed with PCR and the expression of gene -betB in transformants was identified with Western Blot, and the result showed that the transformants were transgenic tobacco plant.

关 键 词：betB基因 遗传转化 表达 烟草 

分 类 号：S572[农业科学—烟草工业]