HPLC法测定山绿茶中芦丁的含量  

Determination the Content of Rutin in Shanlucha by HPLC

作　　者：黎霜[1] 莫蔚明[2] 张治军[1] 刘利敏[1] 卢昕[2] 廖雪英[2] 

机构地区：[1]广西壮族自治区桂林食品药品检验所,桂林541002 [2]广西师范大学化学系

出　　处：《中国药师》2006年第9期807-809,共3页China Pharmacist

摘　　要：目的:建立山绿茶中的芦丁HPLC测定法。方法:使用water C_(18)柱(250 mm×4．6 mm,5μm),以乙晴-2．5％醋酸(14．2:85．8)为流动相,测定波长:254 nm,流速:1 ml·min^(-1),柱温:40℃。结果:芦丁在0．38～3．82μg范围内与峰面积呈良好的线性关系(r=O．999 8),平均回收率98．7％(RSD=2．0％,n=6)。结论:本方法可用于山绿茶的质量控制,方法简便、准确、可靠。Objective： To establish an HPLC method for determination the content of Rutin in Shanlucha. Method： The water C18 column（250 mm ×4.6 mm,5 μm） was used. The mobile phase consisted of Acetonitrile 2.5% acetic acid solution（ 14.2： 85.8）, The detection wave length was 254 nm, flow rate was 1.0ml·min^-1 and column temperature at 40 ℃. Result： the linear range of mtin was 0.38 - 3.82 μg（ r = 0.999 8 ）, the mean recovery of Rutin was 98.7% （ RSD = 2.0%, n = 6）. Conclusion： This method is simple, accurate,and reliable. It can be used to control the quality of Shanlucha.

关 键 词：HPLC 山绿茶 芦丁 

分 类 号：R927.2[医药卫生—药学]