2,5-己二酮对大鼠神经丝mRNA水平的影响  

Effect of 2,5-hexanedione on mRNA levels of neurofilament in rats

作　　者：张天亮[1] 韩晓英[2] 赵秀兰[1] 朱振平[1] 谢克勤[1] 

机构地区：[1]山东大学公共卫生学院毒理学研究所 [2]山东师范大学生命科学学院

出　　处：《毒理学杂志》2006年第4期207-209,共3页Journal of Toxicology

基　　金：国家科技部973重大基础研究资助项目(2002CB512907);国家自然科学基金资助项目(30271138)

摘　　要：目的 探讨2，5-己二酮（HD）对神经组织神经丝（NF）mRNA水平的影响。方法Wistar雄性大鼠27只，分为3组（1个对照组，2个染毒组），每组9只，经腹腔染毒，剂量分别为200和400mg／（kg·d），连续8周。逆转录聚合酶链反应（RT-PCR）方法检测坐骨神经、脊髓、大脑组织中高分子量NF（NF-H）、中分子量NF（NF-M）和低分子量NF（NF-L）mRNA水平。结果 200和400mg／kg剂量染毒后，坐骨神经NF-L mRNA水平分别为对照组的126％（P〈0．05）和152％（P〈0．01），NF-M mRNA未检测到，NF-HmRNA未发生明显变化；在脊髓、大脑组织中，NF-L mRNA下降为对照组的34％-54％（P〈0．01），NF-M mRNA未发生明显变化，400mg／kg剂量组中NF-H mRNA下降为对照组的85％（P〈0．05）。结论 HD染毒导致大鼠神经组织中NF亚单位mRNA水平的变化。Objective To investigate the alterations in mRNA levels of neurofilament （NF） in rats intoxicated with 2, 5- hexanedione（ HD）. Methods HD was administered by intraperitoneal injection to wistar rats with daily dosing of 200 and 400 mg/kg for continuous 8w（five times per week）, mRNA levels of light molecular NF（ NF-L）, medium molecular NF（NF-M） and high molecular NF （NF-H） in sciatic nerve, spinal cord, and cerebrum were determined respectively by RT-PCR. Results mRNA levels of NF-L in rat sciatic nerves were increased to 126% （P 〈 O.05）and 152% （P 〈 0.01 ） at the dosage of 200 and 400 mg/kg individually. For the spinal cord and cerebrum, the expression of NF-L mRNA were both decreased obviously to 34% - 54% （ P 〈 O. 1 ） relatively, and NF- H mRNA of 400 mg/kg dosage were declined to 85 % （P 〈 O. 05 ） of the control. The others were kept relatively unchanged. Conclusion HD-intoxication was associated with alterations in mRNA levels of NF.

关 键 词：2 5-己二酮 神经丝 MRNA RT-PCR 

分 类 号：R993.4[医药卫生—毒理学]