氧乐果致猫脑区Glu和GABA的时间变化  

The temporal change of brain Glu and GABA in omethoate poisoning cats

作　　者：杨艳梅[1] 王立新[1] 刘怀军[1] 李英[1] 周存河[1] 

机构地区：[1]河北医科大学第二医院医学影像科

出　　处：《毒理学杂志》2006年第4期219-221,共3页Journal of Toxicology

基　　金：河北省卫生厅基金项目课题(05131)

摘　　要：目的建立氧乐果皮下染毒急性猫中毒模型,观察急性中毒后脑组织谷氨酸(Glu)和γ-氨基丁酸(GABA)免疫阳性细胞的时空分布规律及形态变化。方法将30只健康猫分为2组。染毒组25只:腹部皮下4点等量注射40%氧乐果0.3 ml/kg(=2.1 mg/kg)。对照组5只:腹部皮下4点等量注射同体积体积分数为0.9%的生理盐水。应用免疫组化的方法观察Glu和GABA免疫阳性细胞的变化。结果免疫组织化学染色观察染毒后顶叶、海马、基底节和小脑Glu阳性细胞数与对照组比较,差异无显著性,顶叶GABA阳性细胞染色加深,阳性细胞数对照组为(9.8±1.2)个/视野,染毒后3h阳性细胞数(14.7±3.3)个/视野,并在染毒后各时间点与对照组比较,差异有显著性。结论氧乐果染毒后顶叶GABA的含量增加。Objective To describe and analyze the temporal change of brain tissue glutamate（Giu） and γ-aminobutyric acid （GABA） immuno-positive cell in cats after omethoate poisoning. Methods 30 healthy cats were divided into two groups. The cats in poisoning group were injected 40% omethoate 0.3 ml/kg （ = 2.1 mg/kg） .The cats in control group were injected 0.9% normal saline 0.3 ml/kg. The Giu and GABA immuno-histochemical staining was analyzed by statistic method in control and poisoning groups. Results The difference between the number of Giu positive cell in control and poisoning group was not significant. After poisoning, the number of GABA positive ceil of occipital lobe was increased from 9.8 ：l： 1.2 in control group to 14.7 =l： 3.3 after 3 h poisoning,and the increase was significant between control group and poisoning groups. Conclusion The quantity of GABA increased in occipital lobe after omethoate poisoning.

关 键 词：氧乐果 中毒 脑 谷氨酸 Γ-氨基丁酸 

分 类 号：R99[医药卫生—毒理学]