纳洛酮对铝致学习记忆减退大鼠海马锥体细胞核酸的影响  

Effect of Naloxone on Nucleic Acid Metabolism in Hippocampal Neurons in Rats with Aluminum-Induced Learning and Memory Impairment

作　　者：孙石磊[1] 吕达平[2] 马光瑜 

机构地区：[1]郑州大学第一附属医院,河南省郑州市450052 [2]安徽中医学院神经病研究所 [3]广州食品药品监督管理局

出　　处：《职业与健康》2006年第19期1528-1530,共3页Occupation and Health

基　　金：广东省自然科学基金资助项目(编号000825)

摘　　要：目的观察纳洛酮对铝所致学习记忆减退大鼠海马锥体细胞内核酸代谢的影响。方法采用慢性氯化铝灌胃方法,制备学习记忆减退大鼠模型后,随机分为模型组和纳洛酮治疗组,另设正常对照组。纳洛酮治疗组大鼠连续7 d腹腔注射纳洛酮。根据荧光探针acridine orange(AO)能与活细胞内DNA及RNA特异性结合后发出不同荧光的特性,利用激光共聚焦显微镜观察海马神经细胞内DNA和RNA的荧光强度,并用图象分析技术进行处理。结果模型组大鼠海马锥体细胞DNA和RNA的平均荧光强度之比为1.78±0.68,比正常组3.84±1.86和纳洛酮组2.97±0.95均明显降低(P<0.05),后两者间差异无显著性(P>0.05)。结论纳洛酮能改善铝致学习记忆减退大鼠海马锥体细胞的核酸代谢。[Objective]To observe the effect of naloxone on DNA and RNA metabolism of hippocampal pyramidal neurons in rats with aluminum- induced learning and memory impairment. [ Methods] Aluminum- induced learning and memory impairment model was established by gavage of altnninum chloride （600 mg/kg） for 3 months. The rats were divided into three groups： naloxone group （NAL 0.8 mg/kg, ip daily for 7 days）, model group and control group. Then laser scanning confocal microscope （LSCM） and Acridine Orange were used to investigate the DNA, RNA changes in hippocampal pyramidal neurons of the three groups. [Results]The fluorescence intensity of DNA/RNA of average foreground per cell in the pyramidal neurons of the model group 1.78 ± 0.68 was significantly lower compared with that of the control group 3.84 ±1.86 and the naloxone group 2.97 ± 0.95 （ P 〈 0.05 ）. No significant difference was found between the control group and the naloxone group （ P 〉 0.05）. [ Conclusion] Naloxone could facilitate the nucleic acid metabolism of pyramidal neurons in rats with aluminuminduced learning and memory impairment.

关 键 词：铝 学习记忆 纳洛酮 核酸 海马锥体细胞 激光扫描共聚焦显微镜 

分 类 号：R96[医药卫生—药理学]