阿托伐他汀对家兔主动脉粥样硬化消退作用的初步观察  

Preliminary observation of regressive effect of atorvastatin on aorta atherosclerosis in rabbits

作　　者：秦玲[1] 杨萍[2] 祝雁[3] 

机构地区：[1]吉林大学第一医院心内科,吉林长春130021 [2]吉林大学第三医院心内科,吉林长春130033 [3]吉林大学前卫医院内科,吉林长春130022

出　　处：《中国现代医学杂志》2006年第16期2440-2443,共4页China Journal of Modern Medicine

基　　金：吉林省科技厅课题(课题编号:20050408)

摘　　要：目的通过高脂饮食诱发家兔主动脉粥样硬化,观察阿托伐他汀对病变局部纤溶酶原激活物抑制物-1(PAI-1)表达的影响及其抗动脉粥样硬化(AS)的作用。方法24只大耳白兔随机分为常规饲料组、胆固醇饲料组和阿托伐他汀组,饲养16周。观察实验前后血清胆固醇(TC)、低密度脂蛋白(LDL)和体质量的变化,处死动物后取主动脉进行病理学检查,采用免疫组化方法检测主动脉壁PAI-1阳性表达百分比。结果16周后阿托伐他汀组血清TC和LDL水平明显低于胆固醇饲料组,分别为(23.51±10.58)mmol/L和(14.27±3.51)mmol/L(P<0.01)、(21.39±10.00)mmol/L和(14.23±4.01)mmol/L(P<0.01)。病理学大体观察,对照组、胆固醇饲料组和阿托伐他汀组斑块/内膜面积比分别为0、0.28±0.37和0.16±0.27,组间比较差异有显著性(P<0.01);光镜下三组内膜厚度分别为4.45±0.58μm、(67.47±7.13)μm和(38.11±6.02)μm、内膜/中膜比分别为(0.0537±0.007)、(0.878±0.370)和(0.391±0.213),组间比较差异有显著性(P<0.01)。免疫组化检测胆固醇饲料组和阿托伐他汀组AS局部PAI-1的阳性表达百分比明显高于对照组(P<0.01);阿托伐他汀组较胆固醇饲料组PAI-1的表达明显减少(P<0.01)。结论阿托伐他汀具有降脂以外的抗AS的作用,抑制PAI-1的过度表达可能是其抗AS的机制之一。[Objective] To determine the effect of atorvastatin on aorta expression of plasminogen activator inhibitor type1-1 （PAI-1）and it/s mechanism of anti-atheroselemsis in athemelerosis rabbits fed with cholesterol-enriched diet. [Methods] Twenty four rabbits were randomly divided into control, cholesterol diet and atorvastatin group, which were fed for 16 weeks. Serum TC, LDL and weigh were measured in 0 and 16 weeks in the three groups. The aortas were harvested for pathologic morphology observation, immunohistoehemistry analysis of PAI-1 positive percentages was performed. [Results] After 16 weeks TC and LDL in atorvastatn group were greatly lower than that cholesterol diet group （P 〈0.01）, they were （23.51±10.58） mmol/L and （14.27±3.51） mmol/L（P 〈0.01）, （21.39±10.00） mmol/L vs （14.23±4.01） mmol/L （P 〈0.01）. The aortas plaque/intima size （P/I） was 0, （0.28±0.37） vs （0.16±0.27） in control, cholesterol diet group and atorvastatn group by pathologic nakey observation （P 〈0.01 ）, intima thickness was （4.45±0.58） μm, （67.47±7.13） μm and （38.11±6.02） μm, intima/medium thickness ratio（I/M） was （0.0537±0.007）, （0.878±0.370） and （0.391±0.213） in the three groups by mieroseope（P 〈0.01）. The expressions of immunohistochemistry analysis of PAI-1 in cholesterol diet group and atorvastatin group were higher than that control（P 〈0.01）, but the expression reduced in atorvastatin group （P 〈0.01）. [Conclusion] Atorvastatin has antiatherosclerosis effect except reducing serum lipids, the inhibition of PAI-1 excessively expression may be one of the anti-atherosclerosis mechanism.

关 键 词：阿托伐他汀 动脉粥样硬化 纤溶酶原激活物抑制物-1 

分 类 号：R543.5[医药卫生—心血管疾病]