干扰素-α2a对流感病毒感染的MDCK作用的研究  

The study of IFN-α2a on MDCK infected with influenza virus A.

作　　者：王欢[1] 吴中明[1] 王美丽[2] 马锐[1] 

机构地区：[1]遵义医学院微生物学教研室,贵州遵义563000 [2]遵义医学院中心实验室,贵州遵义563000

出　　处：《遵义医学院学报》2006年第2期114-115,共2页Journal of Zunyi Medical University

摘　　要：目的探讨干扰素-α2a对甲型流感病毒感染的狗肾传代细胞系(MDCK)的作用。方法采用四甲基偶氮唑蓝(MTT)比色法观察不同浓度(0.01、0.1、1、10、100U/ml)IFN-α2a在H3N2病毒感染MDCK0h、1h和4h后对细胞活性的影响。结果不同浓度的IFN-α2a能抑制正常生长及病毒感染1h、4h后的MDCK的增殖,但能促进病毒感染0h后的MDCK的生长,其中1.0U/ml组与对照组相比有显著差异(P<0.01)。结论IFN-α2a除了可抑制MDCK生长之外,还能促进H3N2感染初期细胞的增殖。Objective To observe the effcts of IFN-α2a on MDCK infected with influenza virus A. Methods To test the effects of different concentrations of IFN-α2a （0.01,0.1,1,10,100U/ml） on MDCK cells at 0,1,4h post-infected by MTT assay. Results The different concentrations of IFN-α2a can inhabit multiplication of normal MDCK cells ,in addition, the cells at 1,4h post-infected were restrained.But it can improve the proliferation of MDCK at Oh post-infected. Conclusions IFN-α2a can stimulate the proliferation of MDCK infected with H3N2 at the early stage besides of inhibiting the growth of MDCK.

关 键 词：干扰素-Α2A 甲型流感病毒 狗肾传代细胞系 

分 类 号：R373.1[医药卫生—病原生物学]