双菌氧化还原耦联转化外消旋2-辛醇制备(R)-2-辛醇  

(R)-2-octanol production from racemic 2-octanol by double strains redox-coupling

作　　者：江娟[1] 徐岩 穆晓清[1] 

机构地区：[1]江南大学生物工程学院教育部工业生物技术重点实验室,江苏无锡214036

出　　处：《化工进展》2006年第8期947-950,962,共5页Chemical Industry and Engineering Progress

基　　金：国家973项目(No.2003CB716000);新世纪人才支持计划(NCET-04-0498);长江学者和创新团队发展计划资助(No.IRT0532)

摘　　要：建立了一种新的制备手性2-辛醇的方法——双菌氧化还原耦联转化外消旋2-辛醇制备(R)-2-辛醇。通过筛选得到了一株选择性氧化外消旋2-辛醇中(S)-2-辛醇的菌株CandidacylindraceaATCC14830,其产物的光学纯度e.e.>98%,(R)-2-辛醇的产率>46%。与报道的一株还原2-辛酮得到(R)-2-辛醇的菌株OenococcusoeniCECT4730氧化还原耦联,底物浓度可达40g/L,产物的e.e.>98%,产率>92%。To develop a practical process for （R）-2-octanol production from racemic 2-octanol, a two step microbial reaction was investigated. The microbial reaction used the eantioselective oxidation of （S）-2-octanol by Candida cylindracea ATCC 14830 and the asymmetric reduction of oxidated 2-octanone by Oenococcus oeni CECT 4730. O. oeni CECT 4730 was a strain reported which can reduce 2-octanone to （R）-2-octanol, C. cylindracea ATCC 14830 was screened as an candidate biocatalyst for preparation of （R）-2-octanol and 2-octanone with the optical purity of 98%e.e. and the yield of 46%. After redox-coupling, the yield and optical purity were 92% and 98% respectively. The substrate concentration was 40g/L.

关 键 词：(R)-2-辛醇 柱状假丝酵母 酒明串珠菌 氧化还原耦联 手性化合物 

分 类 号：Q93[生物学—微生物学]