薄层荧光扫描法测定花生茎中白藜芦醇的含量  

Determination of Resveratrol in Peanut stem by Thin Layer Chromatography Fluorescence Scanning

作　　者：韩小丽[1] 邵鹏[1] 李明静[1] 刘绣华[1] 

机构地区：[1]河南大学化学化工学院,开封475001

出　　处：《天然产物研究与开发》2006年第4期628-630,共3页Natural Product Research and Development

基　　金：河南省教育厅自然科学基金项目(200510475040)

摘　　要：本文采用薄层荧光扫描法测定花生茎中白藜芦醇含量，以氯仿-乙酸乙酯-甲酸（8：2：0．35）为展开剂，狭缝尺寸为3mm×0．45mm，在335nm处进行扫描测定。结果表明：白藜芦醇在46．4～104．4ng范围内线性关系良好，回归方程为：Y=7．446X＋2890．328相关系数R为0．9970。该法简便，快速，精密度高，平均回收率为103．46％，相对标准偏差为0．66％（n=3）。A method was established to determine the content of resveratrol by thin layer chromatography fluorescence scanning. The developing solvent is composed of chloroform,ethyl acetate and formic acid（8：2：0.35） and the slit size is 3 mm×0.45 mm, with the detection wavelength of 335 nm. The results showed that the calibration curves were linear in the range of 46.4 -104.4 ng for resveratrol（R = 0. 9970）. The method has the advantages of simple operation, rapid and accurate. The average recovery is 103.46% and RSD is 0.66%.

关 键 词：薄层荧光扫描法 白藜芦醇 花生茎 

分 类 号：O652.7[理学—分析化学]