异丙酚对大鼠前脑缺血/再灌注后海马Bcl-2表达的影响  

Effects of propofol on hippocampal Bcl-2 protein expression after forebrain ischemia/reperfusion in rats

作　　者：戚思华[1] 韩宝庆[1] 李文志[2] 李军[1] 宋春雨[2] 咸子薇[1] 

机构地区：[1]哈尔滨医科大学第四附属医院麻醉科,150001 [2]哈尔滨医科大学第二附属医院麻醉科,150086

出　　处：《国际麻醉学与复苏杂志》2006年第4期193-196,共4页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金(项目编号:30240023);中国博士后科学基金(项目编号:2003034343);教育部归国人员科研启动基金;华西-宜昌人福药业麻醉学论坛青年医师奖学金(2003年度)

摘　　要：Objective To investigate the effect of propofol on hippocampal Bcl-2 protein expression after forebrain ischemia/ reperfusion (I/R) in rats. Methods forty-two male Wistar rats weighting 250 g^300 g were randomly divided into three groups of fourteen animals: control group (C)in which sham operation was performed; I/R group (I) in which bilateral common carotid arteries were clamped for 10 mins combined with hypotension(MAP:35 mm Hg～45 mm Hg)induced by exsanguinations, then 1 ml/kg saline was injected into the lateral cerebral ventricle using microsyringe; propofol group (P)in which 1 mg/kg propofol was injected into the lateral cerebral ventricle using microsyringe after I/R. The end of 24 h reperfusion the animals were decapitated, then coronal tissue sections and hippocampal tissue were obtained for detection of Bcl-2 protein expression by immuno-histochemistry technique(n = 8)and bcl-2 mRNA expression by RT-PCR technique(n = 6). Results The expression of Bcl-2 protein in group P increased significantly compared with that in group C and I (P < 0.05). The bcl-2 mRNA expression in group P was enhanced compared with that in group C and I (P < 0.05). Conclusion Propofol can enhance the Bcl-2 protein expression and increase the bcl-2 mRNA expression after forebrain ischemia-reperfusion in rats.Objective To investigate the effect of propofol on hippocampal Bcl-2 protein expression after forebrain ischemia / reperfusion （I/R） in rats. Methods forty-two male Wistar rats weighting 250 g - 300 g were randomly divided into three groups of fourteen animals： control group （C）in which sham operation was performed; I/R group （I） in which bilateral common carotid arteries were clamped for 10 mins combined with hypotension（ MAP：35 mm Hg-45 mm Hg）induced by exsanguinations, then ! ml/kg saline was injected into the lateral cerebral ventricle using microsyringe; propofol group （P）in which 1 mg/kg propofol was injected into the lateral cerebral ventricle using microsyringe after I/R. The end of 24 h reperfusion the animals were decapitated, then coronal tissue sections and hippocampal tissue were obtained for detection of Bcl-2 protein expression by immuno-histochemistry technique（ n = 8） and bcl-2 mRNA expression by RT-PCR technique（ n = 6）. Results The expression of Bcl-2 protein in group P increased significantly compared with that in group C and I （ P 〈 0.05）. The bcl-2 mRNA expression in group P was enhanced compared with that in group C and I （P 〈 0.05）. Conclusion Propofol can enhance the Bcl-2 protein expression and increase the bcl-2 mRNA expression after forebrain ischemia-reperfusion in rats.

关 键 词：海马神经元 BCL-2表达 异丙酚 缺血/再灌注 抗凋亡基因BCL-2 半定量逆转录-聚合酶链反应 BCL-2蛋白表达 大鼠 脑缺血再灌注 脑保护作用 

分 类 号：R96[医药卫生—药理学]