反相制备色谱分离酪丝亮肽拟似物  

Purification of Tyroserleutide Peptidomimetics by Reversed Phase Preparative Chromatography

作　　者：刘艺[1] 孙迎基[2] 张奕华[1] 沈子龙[2] 

机构地区：[1]中国药科大学新药研究中心,南京210009 [2]中国药科大学生物技术中心,南京210009

出　　处：《药物生物技术》2006年第4期271-273,共3页Pharmaceutical Biotechnology

摘　　要：本文介绍了以反相C18制备色谱柱分离强极性小肽拟似物的方法。采用BüCHI中压制备系统,以水-甲醇为梯度洗脱体系,并配合HPLC检测,结果成功分离纯化了三个活性拟肽化合物,纯度达97%以上。该法简便、分离效果好,可用于该类小肽化合物的分离制备。A method is described for the separation of strong-polar short peptidomimetics by reversedphase C18 preparative chromatographic column. Bu CHI middle pressure preparative system was applied to automatically control the experiments, using water-methanol as the gradient elution system. The purity of the eluate was validated by HPLC, and three short peptidomimetics were purified which reached 97% purity. The method is simple and effective. It can be applied to separate short peptidomimetics.

关 键 词：反相制备色谱 肽拟似物 

分 类 号：R91[医药卫生—药学]