苏云金杆菌与EoNPV混用的增效作用  

Synergism of Bacillus thuringiensis Combined with EoNPV against Ectropis obliqua

作　　者：徐健[1] 肖强[2] 刘琴[3] 唐美君[2] 殷向东[3] 殷坤山[2] 祝树德[1] 

机构地区：[1]扬州大学农学院,江苏扬州225009 [2]中国农业科学院茶叶研究所,浙江杭州310008 [3]扬州市农业科学研究院,江苏扬州225007

出　　处：《江苏农业学报》2006年第3期243-247,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省科技攻关项目(BE2005612);江苏省农业三项工程项目[SX2003(23)];江苏省农业示范推广项目(BC2003326)

摘　　要：采用生物测定方法测定茶尺蠖核型多角体病毒（Ectropis obliqua nucleopolyhedrovirus，EoNPV）和苏云金杆菌（Bacillus thuringiensis，Bt）的联合作用。5组不同含量EoNPV制剂（1．50×10^7 PIB／ml、1．25×10^7PIB／ml、1．00×10^7PIB／ml、7.50×10^6PIB／ml和5.00×10^6PIB／m1）中混合2000IU／mg Bt制剂后共毒系数在106．02至128．03之间，表明Bt与EoNPV混用具有增效作用。其中以1．00×10^7PIB／ml EoNPV制剂与2000IU／mgBt制剂混用增效作用最明显，LC50为817．03μg/ml。对2龄茶尺蠖的杀虫速度，EoNPV＋Bt制剂（1．00×10^7 PIB／ml EoNPV＋2000IU／mg Bt）的LT50较单独使用EoNPV（2．00×10^7 PIB／ml）缩短了2d，且EoNPV＋Bt制剂对茶尺蠖具有拒食作用，茶尺蠖对EoNPV＋Bt制剂处理茶树的食叶量比单独使用EoNPV制剂的食叶量减少65．9％。EoNPV＋Bt制剂对茶刺蛾、茶银尺蠖等茶树鳞翅目害虫的兼治作用分别达85．8％和88．7％。多点田间药效试验结果表明，EoNPV＋Bt制剂药后10d对茶尺蠖防效达81．80％～93．24％。Synergism of Bacillus thuringiensis （Bt） combined with Ectropis obliqua nucleopolyhedrovirus （EoNPV） against Ectropis obliqua was studied by bioassay in laboratory. The co-toxicity coefficient（CTC） of 2 000 IU/mg Bt mixed with five different concentrations of EoNPV were from 103.02 to 128.03, indicating the existing of the synergistic effects between EoNPV and Bt against E. oblique. Among the five combination treatment, 1.0 × 10^7 PIB/ml EoNPV with 2 000 IU/mg Bt （EoNPV ＋ Bt） was the most synergistic one, with LC50 of 817.03 μg/ml. Compared to the single treatment of EoNPV of 2. 00 × 10^7 PIB/ml,EoNPV Bt treatment not only accelerated the killing speed to the 2nd instar of E. oblique larvae,lessening the median lethal time （LT50） from 11.8 d to 9. 8 d,but also carried the antifeeding activity,resulting in the feeding amount decrease by 65.9%. Field trials in three districts showed that the mortalities of E. oblique ranged from 81.80% to 93.24% after 10-day application of EoNPV Bt,whose dual effects made the mortality of Scopula subpunctaria up to 88.7%.

关 键 词：苏云金杆菌 茶尺蠖核型多角体病毒 茶尺蠖 增效作用 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]