外源一氧化氮对NaCl胁迫下番茄幼苗叶片氧化损伤的保护效应  

Protective Effects of Exogenous Nitric Oxide on Oxidative Damage in Tomato Seedling Leaves under NaCl Stress

作　　者：吴雪霞[1] 朱月林[1] 朱为民[2] 陈建林[2] 刘正鲁[1] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]上海市农业科学院园艺研究所上海市设施园艺技术重点实验室,上海201106

出　　处：《江苏农业学报》2006年第3期276-280,共5页Jiangsu Journal of Agricultural Sciences

基　　金：教育部高校博士点基金资助项目(20030307020);教育部留学回国人员科研启动基金资助项目[(2001)498]

摘　　要：研究了0.05 mmol/L、0.10 mmol/L和0.80 mmol/L的外源NO供体硝普钠(SNP)处理对100 mmol/LNaC l胁迫下番茄(Lycopersicon esculentumM ill.)幼苗叶片氧化损伤的影响。结果表明,在NaC l胁迫处理8 h和24 h时,与100 mmol/L NaC l单独处理相比,0.05 mmol/L和0.10 mmol/L SNP处理均显著提高了番茄幼苗叶片的抗氧化酶SOD、POD、CAT和APX活性以及脯氨酸和可溶性糖含量,显著降低了丙二醛(MDA)和活性氧(O2.-)含量,而0.10 mmol/L SNP处理的效果比0.05 mmol/L处理的效果更好,0.80 mmol/L SNP处理的效果不明显。以上结果表明,外源SNP处理缓解番茄幼苗NaC l胁迫具有剂量效应,0.10 mmol/L SNP处理能显著提高番茄幼苗叶片的抗氧化能力,而且效果最好。The effects of sodium nitropprusside （SNP）, an exogenous nitric oxide donor, at the concentration of 0.05 mmol/L, 0.10 mmol/L and 0. 80 mmol/L on the oxidative damage in tomato （Lycopersicon esculentum Mill. ） seedling leaves under 100 mmol/L NaC1 stress were studied. The results showed that after the treatment of NaCl stress for 8 h and 24 h. SNP at the concentration of 0. 05 mmol/L and 0. 10 mmol/L significantly increased the activities of antioxidant enzymes （ including SOD, POD, CAT and APX） and the contents of proline and soluble sugar in leaves, while significantly decreased malondialdehyde （MDA） content and active oxygen （O2 · - ） producing rate in leaves. SNP concentration of 0. 10 mmol/L was better than that of 0. 05 mmol/L in the alleviation of NaCl stress. However, SNP concentration of 0. 80 mmol/L had no significant effects. The above results indicated that effect of exogenous nitric oxide donor（SNP） existed in a dose-dependent manner in the alleviation of NaCl stress, and the effect of 0. 10 mmol/L SNP was the best of all SNP concentrations in elevating the leaf antioxidation ability of tomato seedlings.

关 键 词：一氧化氮 NACL胁迫 番茄 氧化损伤 

分 类 号：S641.2[农业科学—蔬菜学]