大孔吸附树脂分离纯化异甘草素的研究  

THE STUDY ON SEPARATION AND PURIFICATION OF ISOLIQUIRITIGENIN BY MACROPOROUS ADSORPTION RESIN

作　　者：付玉杰[1] 刘晓娜[1] 侯春莲[1] 赵文灏[1] 祖元刚[1] 施晓光[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《离子交换与吸附》2006年第4期315-322,共8页Ion Exchange and Adsorption

基　　金：教育部新世纪优秀人才支持计划(NCET-04-0316);黑龙江省重点自然基金项目(ZJN04-03);黑龙江省留学回国人员科技活动择优资助经费(黑人函[2005]311号);黑龙江省博士后启动金

摘　　要：研究大孔吸附树脂分离纯化异甘草素的工艺条件及参数。通过研究HPD-600、D4020、D101、AB-8、NKA-II、AL-2和NKA-9树脂对异甘草素的吸附和解吸附能力,筛选最佳树脂为AB-8,并研究了其对异甘草素的吸附和解吸附性能,确定了最佳的吸附与解吸附工艺参数,吸附:pH=5,室温,流速1.5BV/h,溶液处理量为5BV;脱附:洗脱剂为70%的乙醇溶液,流速1BV/h,洗脱剂用量4.5BV。异甘草素样品溶液经AB-8树脂吸附与脱附后回收率为76.7%,纯度由2.02%提高到29.1%,提高了14.4倍。实验结果表明,AB-8树脂对异甘草素的吸附量大,脱附容易,可以应用于异甘草素的分离纯化。The process parameters of the separation and purification of isoliquiritigenin with macroporous adsorption resins were studied. Seven different kinds of macroporous adsorption resins named HPD-600, D4020, D101, AB-8, NKA-Ⅱ, AL-2 and NKA-9 were studied on the static capacity of adsorption and desorption, the better resin was AB-8.The optimum adsorption and desorption parameters of separation and purification isoliquiritigenin with resin AB-8 as follows, adsorption： room temperature, flow rate 1.5BV/h, volume 5BV; desorption： elution solvent 70% alcohol, flow rate 1BV/h, elution volume 4.5BV. After treated with AB-8, the yield of isoliquiritigenin was 76.7%, and the purity was increased from 2.02% to 29.1% with 14.4 times as high as the crude extracts. The results showed that resin AB-8 had good ability of separation and purification of isoliquiritigenin, it could be used to separate and purify isoliquiritigenin.

关 键 词：大孔吸附树脂 异甘草素 分离纯化 吸附 脱附 

分 类 号：O647.3[理学—物理化学]