Toxic Effect of a Pesticide, Diflubenzuron on Freshwater Microinvertebrate (Tetrahymena pyriformis)  

作　　者：ROUABHI Rachid DJEBAR-BERREBBAH H DJEBAR MR 

机构地区：[1]Department of Biology, University Center Larbi Tebessi, Tebessa 12000, Algeria [2]Laboratory of Cell Toxicology, Department of Biology, Faculty of Sciences, Annaba University 23000, Annaba, Algeria

出　　处：《应用与环境生物学报》2006年第4期514-517,共4页Chinese Journal of Applied and Environmental Biology

摘　　要：The impact of diflubenzuron (DFB) on Tetrahymena pyriformis, a ciliated protiste was studied. The treatment with DFB at concentrations of 10 and 20 μg/mL could appreciably reduce the growth of the protiste. The surveillance of protozoa′s respiratory metabolism with polarography technique showed a sensitive inhibition of its oxygen consumption, and at 20 μg/mL concentration the oxygen consumption was increased. For any malformation of morphology and perturbation in swimming trajectory, the neutral red coloration was done. This technique (neutral red) showed that there was a toxic effect of DFB on T. pyriformis, especially at the highest concentration; this toxicity was translated by rotatory swimming in same place and the increasing of coloration quantity in digestive vacuoles. Fig 5, RefThe impact of diflubenzuron （DFB） on Tetrahymena pyriformis, a ciliated protiste was studied. The treatment with DFB at concentrations of 10 and 20 μg/mL could appreciably reduce the growth of the protiste. The surveillance of protozoa＇s respiratory metabolism with polarography technique showed a sensitive inhibition of its oxygen consumption, and at 20μg/mL concentration the oxygen consumption was increased. For any malformation of morphology and perturbation in swimming trajectory, the neutral red coloration was done. This technique （ neutral red） showed that there was a toxic effect of DFB on T. pyriforrnis, especially at the highest concentration; this toxicity was translated by rotatory swimming in same place and the increas- ing of coloration quantity in digestive vacuoles.

关 键 词：Tetrahymena pyriformis ciliated protiste DIFLUBENZURON polarographic technique PESTICIDE toxicity test respiratory metabolism 

分 类 号：Q959.11[生物学—动物学]