染色体定位粗山羊草抗小麦白粉病基因PmAeY1  

Chromosomal Localization of Powdery Mildew Resistance Gene PmAeY1 from Ae.tauschii Y219

作　　者：张海泉[1] 贾继增[1] 张宝石[2] 周荣华[1] 

机构地区：[1]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081 [2]沈阳农业大学农学院,辽宁沈阳110161

出　　处：《中国农业科技导报》2006年第4期19-22,共4页Journal of Agricultural Science and Technology

基　　金：科技部973项目"小麦重要新基因的发掘与有效利用研究"资助(G1998010205)

摘　　要：小麦白粉病是严重影响小麦生产的重要病害之一,利用抗病品种是防治该病最为经济、有效和环境安全的方法。目前已经标记31个小麦抗白粉病基因,但大多数抗性丧失或与不良性状紧密连锁。粗山羊草存在许多小麦抗病基因,它可以扩大小麦抗病基因的基础,提供新的抗小麦白粉病基因的来源。使用分离群体分组分析法(BAS),将抗小麦白粉病E11菌株的粗山羊草材料Y219与感病材料Y169杂交,F1代表现抗病,F2代出现抗感3∶1分离,用SSR标记技术,抗病新基因PmAeY1定位在2D染色体上,与Xgwm484、Wmc453、Xgwm515和Xgwm157的遗传距离分别是30.4、23.4、6.1和5.5cM。Powdery mildew is one of the devastating diseases of wheat. The use of resistant cultivars is the most economical, effective and environmentally safe way to control this disease. Thirty-one genes resistant to powdery mildew have been found, but most of them have lost resistance function and application value. The Ae. tauschii contains plentiful resistance genes, which will enlarge the disease-resistance genetic foundation and improve resistance breeding. Based on the populations derived from cross between Ae. tauschii Y219（resistant） and Y169（ susceptible）, F1 representatives are disease-resistant and the ratio of resistance/susceptible was 3：1 in F2 generation. Using bulked segregant analysis （BAS）, 200 SSR markers on wheat D gemone were screened. Xgwm484,WMC453,Xgwm515 and Xgwm157 on chromosome 2D were linked to powdery mildew resistance and the genetic distance were 30.4cM,23.4 cM,6.1 cM and 5.5cM respectively. The new resistance gene was named PmAeyl.

关 键 词：粗山羊草 小麦白粉病 抗病基因 SSR标记 

分 类 号：Q755[生物学—分子生物学]