间硝苯地平对血管紧张素Ⅱ促进血管平滑肌细胞增殖和蛋白质合成的影响  

INHIBITORY EFFECTS OF M-NIFEDIPINE ON ANGIOTENSIN ⅡSTIMULATED PROLIFERATION AND PROTEIN SYNTHESIS IN CULTURED RABBIT AORTIC SMOOTH MUSCLE CELLS

作　　者：古宏标[1] 汤聿海[1] 徐毅 

机构地区：[1]广东药学院病理生理教研室,湖南医科大学药理教研室,湖南医科大学心血管生理室

出　　处：《药学学报》1996年第10期732-736,共5页Acta Pharmaceutica Sinica

摘　　要：以培养血管平滑肌细胞（ｖａｓｃｕｌａｒｓｍｃｏｔｈｍｕｓｃｌｅｃｅｌｌ，ＶＳＭＣ）为模型，观察了间硝苯地平（ｍ－ｎｉｆｅｄｉｐｉｎｅ，ｍ－Ｎｉｆ）对血管紧张素Ⅱ（ａｎｇｉｏｔｅｎｓｉｎⅡ，ＡＮＧⅡ）促进ＶＳＭＣ增殖和蛋白质合成的影响。结果表明，ｍ－Ｎｉｆ抑制ＡＮＧⅡ（１００ｎｍｏｌ·Ｌ￣（－１））引起ＶＳＭＣ［￣３Ｈ］ｔｈｙｍｉｄｉｎｅ和［￣３Ｈ］ｌｅｕｃｉｎｅ参入，并呈剂量依赖性。ｍ－Ｎｉｆ（２×１０￣（－６）ｍｏｌ·Ｌ￣（－１））可抑制ＡＮＧⅡ对ＶＳＭＣ的刺激、ＤＮＡ及蛋白质合成速率，分别降低了４６％，５８％，５３％。By using rabbit aortic smooth muscle cells(VSMC)in culture as a model,theeffects of m-nifedipine(m-Nif)on the growth of VSMC were observed.The results showed that m-Nif inhibited [3H]-thymidine and[3H]-leucine incorporation into DNA and protein,ecreased cellnumber in a concentration-dependent manner in angiotensinⅡ(ANGⅡ)induced VSMC.At theconcentration of 2 μmol·L-1,m-Nif was found to reduce the ANGⅡ(100 nmol·L-1)stimulated[3H]-thymidine,[3H]-leucine incorporation and cell number by 46%,58% and 53%,respectively。Our results suggest that m-Nif can inhibit ANG Ⅱ stimulated proliferation and protein synthesis incultured rabbit aortic VSMC

关 键 词：间硝苯地平 血管紧张素Ⅱ 血管平滑肌细胞 

分 类 号：R972[医药卫生—药品]