水稻矮缩病毒云南、浙江分离株侵染水稻植株的细胞病理比较研究  

The comparative studies on the cytopathology of rice plants infected by Rice dwarf virus Yunnan and Zhejiang isolates

作　　者：张仲凯[1] 方琦[1] 魏春红[2] 丁铭[1] 董家红[1] 俞立[2] 李毅[2] 

机构地区：[1]云南省农业生物技术重点实验室,云南昆明650223 [2]北京大学生命科学学院,北京100871

出　　处：《西南农业学报》2006年第4期621-623,共3页Southwest China Journal of Agricultural Sciences

基　　金：国家重点基础研究发展规划("973"计划)资助项目(No.G2000016201)

摘　　要：应用超薄切片电镜技术比较观察RDV云南和浙江分离株侵染水稻植株的细胞病理。RDV云南分离株侵染的水稻叶片中,充满病毒粒体的细胞呈条带状分布,呈现出明显的侵染和增殖中心,在充满病毒粒体的细胞周围,相邻细胞有不同程度的病变,叶绿体膜系统消失,部分叶绿体中含有病毒颗粒,线粒体残体及细胞质中有分散或聚集的病毒粒体,细胞核中未见病毒粒体,离侵染中心较远的细胞中亚细胞结构较完整;RDV浙江分离株侵染的水稻叶片中,部分细胞充满病毒粒体,无明显的条带状分布,其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。RDV不同分离株侵染寄主植株的细胞病理特征无显著差异,对其致病性的差异需进一步研究。Costive observation of the cytopathology of rice plants infected respectively by RDV Yurman and Zhejiang isolates,using ultrasection and TEM （Transmission electron microscope） techniques. In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells full of virus particles were some cells with the different extent of lesion as follows： disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some single or assembled virions in the mitochodria or cytoplasma but not in nuclei,and intact subcellular structure in the cell far away from the infected center; in the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn＇ t form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.The distinct cytopathological characters in the cells infected by different isolates were not observed, and more research on the pathogenic variation should be worked on.

关 键 词：水稻缩病毒 细胞病理 侵染中心 致病性 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]