放线菌次生代谢产物对百合灰霉病菌的抑菌活性筛选  

Primary study on inhibition activity of actinomycetes seconday metabolites to Botrytis elliptica(Berk.) Cooke.

作　　者：刘树芳[1] 李艳琼[2] 曾莉[1] 李铭刚[2] 杨佩文[1] 李一青[2] 尚慧[1] 崔晓龙[2] 李家瑞[1] 文孟良[2] 

机构地区：[1]云南省农业科学院农业环境资源研究所,云南昆明650205 [2]云南大学教育部微生物资源开放研究重点实验室云南省生物资源保护与利用重点实验室,云南昆明650091

出　　处：《西南农业学报》2006年第4期627-630,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金重点项目"微生物杀植物病原真菌制剂的应用基础研究"(2004C0002Z)资助

摘　　要：以268份放线菌次生代谢产物为筛选对象,百合灰霉病为供试菌种,通过抑制孢子萌发试验、黄瓜子叶法两种试验方法,进行抗百合灰霉病活性筛选。结果表明,孢子萌发抑制率80%以上的有16份,黄瓜子叶离体叶片防治效果50%以上的有10份。筛选到一批具有开发应用前景的放线菌资源。268 actinomycete isolates were gained from soil samples, which were sampled from different places in Yunnan. On the basis of antagonistic examination of inhibition effect on conidia germination and in vitro leaves of cucumber control efficiency, the actionmycete strains inhibit to B. elliptica were selected. The results showed that of 268 strains, the control efficiency of 10 strains had protective activities above 50 %, the coniidia germination inhibition rate of 16 strains were above 80 % .These strains may have poteafial to develop as fungicide to control plant disease.

关 键 词：放线菌 次生代谢产物 百合灰霉病 活性测定 

分 类 号：S644.1[农业科学—蔬菜学]