采用RT-PCR技术检测甜樱桃“红灯”上的PNRSV  

Detection of PNRSV in sweet cherry varity HongDeng' by RT-PCR

作　　者：侯义龙[1] 张立娟[1] 于亚军[1] 于大永[1] 

机构地区：[1]大连大学生物工程学院,辽宁大连116622

出　　处：《大连大学学报》2006年第4期59-60,共2页Journal of Dalian University

基　　金：辽宁省博士启动基金项目(20021052);辽宁省教育厅高等学校科研项目(2004D202)

摘　　要：[目的]调查大连市特色水果品种甜樱桃“红灯”的田间植株携带PNRSV的情况．[方法]利用作者已建立的PNRSV RT-PCR检测体系．[结果]被检植株PNRSV的感染率达100％,但程度不同．[结论]对于其中感染PNRSV较轻的植株可以作为今后培育无PNRSV苗木的原材料．[ Objective] To investigate whether the sweet cherry varity＂ gDeng＂ in hard was infected by PNRSV or not. [ Methods] The RT-PCR detection system published for PNRSV was used. [ Results] All the trees detected were infected by PNRSV,but the infected degree was different. [ Conclusion] The trees which were not infected seriously will be chosen as the primary materials to culture the young plants with PNRSV-free.

关 键 词：逆转录-聚合酶链式反应 PNRSV 检测 

分 类 号：Q94[生物学—植物学]