苦参不同器官中的生物碱含量分析  

Analysis of alkaloid content in different organs in Sophora flavescens

作　　者：任欣[1] 于昉[1] 闫建义[2] 赵东利[1] 

机构地区：[1]大连大学医学院,辽宁大连116622 [2]大连大学动物中心,辽宁大连116622

出　　处：《大连大学学报》2006年第4期79-81,共3页Journal of Dalian University

摘　　要：[目的]探讨苦参不同器官中的生物碱含量，为该资源植物的合理应用提供依据．[方法]用薄层色谱法（TLC）分析测定了辽宁产苦参不同器官中苦参碱及氧化苦参碱含量．[结果]各类器官，除叶片外，氧化苦参碱含量均显著超过苦参碱含量；苦参碱含量以种子中的为最高，占干重的0．065％，其次为茎（0．034％）、根（0．016％）、叶（0．013％）；氧化苦参碱含量以根最高，占干重的3．847％，其次为种子（1．982％）、茎（0．140％），叶中未检测到；粗老根（直径大于1cm）与细嫩根（直径在0．5-1．0cm）中的两种生物碱含量差别不大．[结论]达到一定生长年限后，生物碱积累不再有明显增加而趋于饱和；大部分器官中，氧化苦参碱含量高于苦参碱含量；种子及茎更适合作为苦参碱的提取器官．[ Objective ] In order to right use Sephora flavescens plant resource, contents of matrine and oxymatrine of various organs of Sophora flavescens from Liaoning province were tested in the present paper. [ Methods] TLC method. [ Results] Matrine of seeds is the highest in content among all the tested organs,being up to 0. 065% of dry weight,then stems （0. 034% ） ,roots （0. 016% ） and leaves （0. 013% ） ;The content of oxymatrine in roots is the highest, up to 3. 847% of dry weight, and then seeds （ 1. 982% ） and stems （ 0. 140% ）, no oxymatrine was detected in leaves. The alkaloid content is not significantly different between old rough roots more than 1.0cm in diameter and young thin ones of 0.5 - 1.0 cm in diameter. [ Conclusions ] Contents of alkaloids in Sophora flavescens plants will keep stabilization in mature plants although the plants still grow;Oxymatrine is more than matrine in most organs of Sophora flavescens ; Seeds and stems are appropriate organs for matrine extraction.

关 键 词：苦参 苦参碱 氧化苦参碱 薄层层析 含量分析 

分 类 号：R942.6[医药卫生—药剂学]