反相离子对高效液相色谱法测定血浆吗啡及其代谢物M3G的含量  

DETERMINATION OF MORPHINE AND METABOLITE M3G IN RAT PLASMA BY REVERSED-PHASE IRON-PAIR HPLC

作　　者：贺瑞英[1] 刘丽京[1] 邓艳萍[1] 

机构地区：[1]北京大学中国药物依赖性研究所,北京100083

出　　处：《中国药物依赖性杂志》2006年第4期272-276,共5页Chinese Journal of Drug Dependence

基　　金：国家重点基础研究发展计划(973)项目(2003CB515400)资助

摘　　要：目的:建立反相离子对高效液相色谱法测定血浆吗啡及其代谢物3-葡萄糖醛酸吗啡(M3G)的浓度。方法:血浆样品以乙酸乙酯提取,以纳洛酮为内标。应用HPLC-UV方法测定吗啡浓度,分析柱DiamonsilTMC18(250mm×4.6mm),流动相为0.01mol·L-1磷酸二氢钾缓冲液(含0.5mmol·L-1的十二烷基磺酸钠)-乙腈(71:29),检测波长210nm,流速0.65ml·min1。血浆样品经β-葡萄糖醛酸苷酶水解测定其代谢物含量。结果:吗啡标准曲线线性范围宽(10-4000μg·L-1),线性关系良好;最低检测限10μg·L-1;高、中、低浓度的回收率在85·3%以上。血浆M3G在250-8250μg·L-1浓度范围内与水解增加吗啡浓度呈线性关系。结论:本法简单,快速,血浆中杂质不干扰样品的测定,满足生物样品分析要求。Objective： To establish a reversed - phase iron - pair HPLC for determination of morphine and metabolite morphine- 3 -glucuronide （M3G） in rat plasma. Methods： Morphine was extracted from rat plasma using ethyl acetate, then determinated by HPLC - UV after separation on a DiamonsilTM Cis analytical column （250 mm × 4.6 mm ）, The mobile phase was 0.01 mol · L^-1 potassium dihvdrogen Dhosphate （0. 5 mmol·L^-1 sodium dodecvlsulfonate） - acetonitrile （71： 29）,thewavelength of detection was set at 210 nm, and flow rate was 0. 65 ml· min^-1 Internal standard was naloxone. Plasma was hydrolyzed by β -glueuronidase for determination metabolite M3G. Results： Linearity was verified from 10 to 4000 μg· L^-1, the limit of quantition（LOQ） was 10 μg· L^-1 . The recovery of morphine was above 85.3%. Linearity was also verified between the concentration of M3G （from 250 to 8250 μg· L^-1） and morphine obtained from M3G in the plasma. Conclusion：This method is simple, rapid and accurate for quantitative analysis of morphine in rat plasma.

关 键 词：高效液相色谱法 吗啡 3-葡萄糖醛酸吗啡 

分 类 号：R978.19[医药卫生—药品] R446.11[医药卫生—药学]