人参皂甙Rh_2体外对小鼠黑色素瘤细胞的分化诱导作用  

DIFFERENTIATION OF B_(16) MELANOMA CELLSINDUCED BY GINSENOSIDE RH_2

作　　者：夏丽娟[1] 韩锐[1] 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所

出　　处：《药学学报》1996年第10期742-745,共4页Acta Pharmaceutica Sinica

摘　　要：体外实验证明人参皂甙Ｒｈ＿２在１０μｇ·ｍｌ￣（－１）的浓度下能明显抑制Ｂ＿（１６）细胞的生长，并呈浓度依赖性，其ＩＣ＿（５０）为４．１μｇ·ｍｌ￣（－１）。Ｒｈ＿２在１０μｇ·ｍｌ￣（－１）浓度下对Ｂ＿（１６）细胞有较强的分化诱导作用，表现为黑色素生成能力明显增加；形态向上皮样细胞分化；细胞呈网状结构；黑色素颗粒增多，生长变缓慢。细胞动力学研究结果表明，Ｒｈ＿２可使Ｂ＿（１６）细胞阻断在Ｇ＿１期。The effect of ginsenoside Rh2,a constituent isolated from Panax ginseng C.A.Meyer,on the growth of tumor cells in vitro was investigated.The results showed that Rh2 inhibitedthe growth of B16 cells at the concentration of 10μg·ml-1(IC50:4.1μg·ml-1).Rh2 was found tosignificantly induce the activity of differentiation of B16 cells at the concentration of 10μg·ml-1 invitro。The melanin synthesis of Rh2 in treated B16 cells was increased.Morphologically,the Rh2induced B16 cells turned to be epithelioid cells.B16 cells became dendrite shaped morphologically athigher concentration of Rh2.Flow cytometry demonstrated that the B16 cells treated with Rh2 wereblocked at G1 phase.

关 键 词：人参皂甙Rh_2 分化诱导 B_(16)黑色素瘤 

分 类 号：R285.5[医药卫生—中药学]