声损伤后基底乳头中DNA复制与细胞增殖  

DNAreplicationandcelularproliferationinthenoisedamagedbasilarpapilarofchicks

作　　者：孙建军[1,2] 汪吉宝[1,2] 李桂榕 

机构地区：[1]海军总医院耳鼻咽喉科 [2]同济医科大学协和医院耳鼻咽喉科

出　　处：《中华耳鼻咽喉科杂志》1996年第6期344-347,共4页Chinese Journal of Otorhinolaryngology

摘　　要：为探讨听觉上皮修复过程的细胞生物学特点，用剂量为１１５ｄＢ（Ａ）×７２小时的宽带噪声建立８～１４日龄鸡声损伤模型。于不同时间用ＤＮＡ合成示踪剂——氚标记胸腺嘧啶核苷（３Ｈ－ＴｄＲ）予以标记。取基底乳头连续培养并进行放射自显影观察。实验显示，首次标记４８小时后受损区可见标记阳性细胞（或前体细胞）。标记毛细胞出现于９６小时。５～６天时，标记毛细胞处于分化过程中。对照组未见标记毛细胞。结果表明，声损伤后听觉上皮存在着ＤＮＡ的复制与细胞增殖，３Ｈ－ＴｄＲ标记阳性细胞最终定位于基底乳头表层并分化为毛细胞。提示基底乳头局部组织中的某些支持细胞为再生毛细胞的前体。Anacoustictraumamodelforstudyoftherepairoftheauditoryepitheliawasestab-lishedinpostnatal8～14dayschicks.Theanimalswerecontinuouslyexposedtothewidebandnoiseat115dB(A)for72hours.ThetracerofDNAreplication-[3H]thymidine(3H-dR)wasgiveninvivoorinvitroduringorafterexposure.Thebasilarpapilawereculturedandprocessedwithautoradio-graphictechnique.Theresultsdemonstratedthatat48hoursfolowinginitiallabelingoftracer,theincorporationof3H-TdRwasfoundoverthenucliofsupportingcels(orprecursorcels)intheinjuredregionofbasilarpapila.Thelabeledhaircelsappearedat96hoursnearthelabeledsup-portingcels.Untilthefifthtosixthdaysofculturing,theregeneratedhaircelswerestilimmaturemorphologicaly.Nosignoflabeledhaircelsweredetectedinthecontrolanimals.Ourfindingsin-dicatedthepotentialcapabilityofcelularproliferationandDNAreplicationindamagedauditoryep-itheliainvitro.Somekindofsupportingcelsindamagedregioncouldbetheprecursorsforregener-atinghaircels.Thepositivecelslabeledwith3H-TdRmigratedtothesurfaceofbasilarpapilaranddiferentiatedintohaircelseventualy.Therefore,itcouldbespeculatedthatahomogeneousre-lationshipmayexistbetweenlabeledsupportingcelsandlabeledhaircels.

关 键 词：毛细胞 听力丧失 细胞增殖 DNA 修复 

分 类 号：R764.5[医药卫生—耳鼻咽喉科]