具有细胞毒活性红树林真菌094811的鉴定  

Identification of Mangrove(Rhizophora) Fungi Strain 094811 Possessing Cytotoxic Activities

作　　者：张志华[1] 洪葵[1] 高昊 姚新生 

机构地区：[1]中国热带农业科学院热带作物生物技术国家重点实验室,海南海口570110 [2]深圳市创新中药及天然药物重点实验室,广东深圳518055

出　　处：《微生物学杂志》2006年第4期6-11,共6页Journal of Microbiology

基　　金："863"海洋生物技术项目(2004AA628040);科技部社会公益项目(004DIB3J072)

摘　　要：为鉴定合成细胞毒活性及多羟基甾醇脂肪酸酯新结构代谢产物的红树林真菌094811,采用形态观察、BIOLOG生理特征鉴定及核糖体18S rDNA测序对菌株094811进行了鉴定。菌株094811与模式菌株黑曲霉As3.40、泡盛曲霉As3.44形态特征极为相似,难以区分。扫描电镜下分别观察且比较菌株094811、模式菌株黑曲霉As3.40、泡盛曲霉As3.44分生孢子的形态,判断菌株094811为泡盛曲霉;根据BIOLOG微生物鉴定系统给出的相似菌种名称及综合形态和生理生化特征,推断菌株094811为泡盛曲霉;通过18S rDNA部分序列测序、GenBank比对,及系统演化分析进一步确认菌株094811为泡盛曲霉。菌株094811的形态、生理特征及其核糖体18S rDNA的序列特征给出了一致的结论。首次鉴定发现1株具有合成细胞毒活性及新的多羟基甾醇脂肪酸酯结构化合物的能力的泡盛曲霉。Three methods were used in order to identify mangrove （Rhizophora） fungal strain 094811 with its cytotoxic activities and new structure metabolite of polyhydroxysterol fatty acid esters produced by the strain, they were morphological observation, BIOLOG physiological identification, and 18S rDNA sequence. Strain 094811 was very similar model strain of Aspergillus niger As 3.40 and A. awamori As3.44 and very difficult to distinguish their differences. Spore morphology of 094811, As. 3.40, and As 3.44 were observed and compared under scanning electron microscope, and determined that 094811 was A. awamori, and according to similar strain names and properties of synthetical morphology, physiology, and biochemistry given by microbial identification system of BIOLOG, it was deduced ＂chat 094811 was A. awarnori. 18S rDNA sequence and GenBank blast also confirmed that 094811 was A. awamori. Morphology, physiological features, and 18S rDNA sequence of ribosome of 094811 gave identical conclusion. Thus it was the first identification report on a strain of Aspergillus awamori possessing cytotcoxic acitivities and produced polyhydroxysterol fatty acid esters.

关 键 词：泡盛曲霉 细胞毒活性 18S RDNA 菌种鉴定 

分 类 号：Q939.5[生物学—微生物学]