高效液相色谱法测定二甲双胍血药浓度  

Determination of Metformin Plasma Concentration by HPLC

作　　者：冯鸣燕[1] 李俊杰[1] 陈琳[1] 杨杲岚[1] 

机构地区：[1]暨南大学附属第一医院药剂科,广州510632

出　　处：《江西医学院学报》2006年第4期52-54,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的建立二甲双弧的高效液相色谱法及其片剂的血药浓度测定方法。方法采用高效液相色谱法测定二甲双胍血药浓度。色谱柱为Hypersil ODS（250mm×4．6mm，5μm），流动相为水。乙腈（70：30，内含0．014moL／L磷酸氢二胺，0．003moL／L十二烷基硫酸钠，加冰醋酸调pH值为6．8），流速为1．2mL／min，检测波长232nm。结果二甲双胍血药浓度为50～2000μg／L，浓度与峰面积比线性良好，相关系数r=0．9990，最低检测浓度为50μg／L。高中低浓度样品的日内、日间测定RSD均〈10％，绝对回收率均〉70％。结论本方法简便、灵敏、准确、可靠，可作为二甲双胍血药浓度的测定方法。Objective To establish a HPLC method to measure the plasma concentration of metformin. Methods A Hypersil-ODS column （250 mm×4.6mm,5μm） was selected. Mobile phase was a mixture of water and acetonitrile （70 ： 30, v/v,0. 014 moL/L（NH4）2HPO4, 0. 003 moL/L CH3（CH2）11SO4Na,pH=6.8） with flow rate of 1.2 mL/min. The detection wavelength was 232 nm. Results The standard curve was linear over the range of 50-2 000 μg/L of metformin in plasma （r=0.999 0）. The limit of detection was 50μg/L. The recovery rate was more than 70% and the RSD within a day and between days did not exceed 10%, respectively. Conclusion The method is simple, sensitive, accurate and reliable which can be used in the pharmaco- kinetics study of metformin.

关 键 词：高效液相色谱法 二甲双胍 血浆 药物浓度 

分 类 号：R927.1[医药卫生—药学]