Eu（Ⅲ）-TMA体系的荧光增强及其在水稻代谢中的应用研究  

Fluorescence Enhancement of Eu（Ⅲ） - TMA System and Eu（Ⅲ） Metabolism in Rice

作　　者：赵学辉[1] 刘志国[1] 彭絮孟[1] 

机构地区：[1]株洲师范高等专科学校化学化工系,湖南株洲412007

出　　处：《株洲师范高等专科学校学报》2006年第5期33-37,共5页Journal of Zhuzhou Teachers College

基　　金：湖南省教育厅科研资助项目（01C030）

摘　　要：在溶液的pH为6．0，均苯三甲酸（TMA）的浓度为2．0×10^-1mol·L^-1时．Eu^3＋-TMA体系可使Eu^3＋的荧光强度增大三个多数量级．光学惰性稀土La^3＋与Lu^3＋，在浓度分别为2．0×10^-4mol·L^-1、1．5×10^-4mol·L^-1时，可使Eu^3＋-TMA体系荧光强度分别增大178．1与91．3倍．体系中Eu^3＋的浓度在4．7×10^-8～2．3×10^-5mol．L^-1的浓度范围内与体系的荧光强度呈线性关系．Eu^3＋的检出限达到了1．0×10^-8mol·L^-1．用此优化发光体系研究铕（Ⅲ）在水稻不同生育期被水稻吸收后的分布定位情况．结果表明：铕（Ⅲ）进入水稻后与水稻各部位的结合不是随机的．主要是在分蘖期与水稻的叶片结合．为稀土元素在水稻中的代谢研究提供了一种可借鉴的方法与研究手段．The fluorescence of europium（Ⅲ） was enhanced by about three orders of magnitude in the presence of 2.0 10^-4mol · L^-1TMA（trimesic acid） in aqueous solution at pH 6. The fluorescence intensity could be greatly increased when the system of Eu^3＋-TMA was treated with La^3＋ and Lu^3＋. The addition of La^3＋ can enhance the fluorescence of the system by above two orders of magnitude, when its concentration is 2.0× 10^-4mol · L^-1. In the optimum conditions, the fluorescence intensity of the system and the concentration of Eu^3＋ have a linear function relationship when the concentration of Eu^3＋ is in the range of 4.7 × 10^-8 -2.3× 10^-5mol · L^-1 and the limit of detection is 1.0× 10^-8 mol· L^-1. This system was also applied to analyzing the content of Eu（Ⅲ） absorbed by rice root, stem and leave in different stages. The results show that the content of Eu（Ⅲ） in rice root,stem and leave is different, and the content of Eu（Ⅲ） in leave in rice parturition stages is highest. A subsidiary method has been developed for studying the metabolism of lanthanides in rice.

关 键 词：铕 均苯三甲酸 水稻生育期 荧光增强 

分 类 号：O657.31[理学—分析化学]