胶原-壳聚糖载硫酸长春新碱微球缓释药膜的研究  

Preparation and characteristics of collagen-chitosan complex film with embedded PLGA microspheres

作　　者：陈红丽[1] 陈汉[1] 莫丽都尔[1] 刘玲蓉[1] 张其清[1] 

机构地区：[1]中国医学科学院中国协和医科大学生物医学工程研究所天津市生物医学材料重点实验室,天津300192

出　　处：《国际生物医学工程杂志》2006年第4期193-196,共4页International Journal of Biomedical Engineering

基　　金：国家863计划课题资助项目(2002AA326040)

摘　　要：目的本研究制备载硫酸长春新碱(vincristinesulfate,VCR)微球的胶原-壳聚糖缓释药膜。并考察加入壳聚糖对药膜性质的影响。选定适当的胶原壳聚糖比例制备药膜。方法采用W/O/O溶剂挥发法制备VCR的聚乳酸-羟基乙酸(poly(lactic-co-glycolicacid),PLGA)微球,并对微球性质表征,采用二次冻干法制备载VCR微球的胶原-壳聚糖药膜,对药膜的表面形态、降解性质、热力学性质及释放性质进行表征,并与释放2周后的药膜进行比较。采用高效液相法分析药物含量。结果VCR制成PLGA微球后再制备成药膜,可达到双重缓释的作用,明显减少药物突释,并延缓药物释放。添加了壳聚糖的药膜降解速度明显小于单纯的胶原药膜。在体外释放实验中,微球突释为(27.2±1.2)%,而胶原药膜的突释为(20.4±1.9)%,胶原与壳聚糖比例为9∶1、4∶1、3∶2的药膜突释分别为(20.2±2.1)%、(18.0±1.1)%和(16.3±1.8)%。结论胶原壳聚糖载VCR的缓释药膜能不同程度减少药物的突释,使药物释放更加平稳缓慢,优于单纯的胶原药膜。Objective The objective of this study is to investigate a collagen-chitosan complex film with embedded vincristine sulfate （VCR） microspheres （MSs）. Methods A water-in-oil-in-oil double-emulsion/solvent evaporation method was used to prepare VCR-loaded PLGA microspheres. The microspheres were further mixed with collagen and/or chitosan swelling solution, followed by lyophilizing. The films were cross-linked by using 0.3% glutaraldehyde. Encapsulation efficiency, release kinetics of VCR microspheres, release kinetics as well as in vitro degradation of the film were evaluated. Results VCR was released from the film in a prolonged period and the initial burst release was less significant. The initial release was （27.2±1.2）% for the naked MSs. After the MSs were embedded in the films of collogan and collagen-chitosan （9：1,4：1,3：2, v/v）, the burst release decreased to （20.4±1.9）%,（20.2±2.1）%,（18.0±1.1）%,（16.3±1.8）%, respectively. In the degradation experiment, the film containing chitosan degraded more slowly than that of without chitosan. Conclusion The collagen-chitosan complex film could achieve the release kinectics of a relatively constant release.

关 键 词：硫酸长春新碱 聚乳酸-羟基乙酸 微球 胶原 壳聚糖 

分 类 号：R94[医药卫生—药剂学]