RP-HPLC同时测定蔬菜中甲基对硫磷和对硫磷的残留量  

Simultaneous detection by RP-HPLC of methyl-parathion and parathion residue levels in vegetables

作　　者：王绍云[1] 周光明[2] 王莉[2] 李蓉[2] 

机构地区：[1]凯里学院应用化学研究所,贵州凯里556000 [2]西南大学化学化工学院,重庆400715

出　　处：《农药》2006年第9期622-623,共2页Agrochemicals

基　　金：重庆市自然科学基金资助(CSTC-2004BB7087)

摘　　要：建立了同时测定甲基对硫磷、对硫磷在蔬菜中残留量的反相高效液相色谱分析方法。用HypersilC18柱(4.6mm×300mm,5μm)色谱柱,以甲醇∶水(60∶40,v∶v)为流动相,流速1.0ml/min,270nm检测。甲基对硫磷、对硫磷分别在0.04￣10.00、0.002￣10.00μg/ml范围内呈良好的线性关系,检出限分别为0.036、0.00043μg/ml,两种农药(添加浓度0.05、0.5、2μg/g)的回收率为91.8%￣94.8%,相对标准偏差为1.06%￣2.41%。A method was developed for simultaneous determination of methyl-parathion and parathion residues in vegetables by reversed-phase high performance liquid chromatography. A Hypersil C 18 column （4.6 × 300 mm, 5μm） with CH3OH：H2O （60：40, by vol） as the mobile phase, a flow rate of 1.0 ml/min, and an ultraviolet detection wavelength of 270 nm. Methyl-parathion and parathion showed linear responses in the concentration ranges of 0.04 to 10.00 and 0.002 to 10.00 μg/ml, respectively, and detection limits of 0.036 and 0.00043 μg/ml, respectively. Recoveries of the two pesticides （total additive concentrations of 0.05, 0.5, and 2 μg/g） were 91.8 to 94.8% with relative standard deviations （RSD） from 1.06 to 2.41%.

关 键 词：RP—HPLC 甲基对硫磷 对硫磷 农药残留 蔬菜 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] TS207.53[轻工技术与工程—食品科学与工程]