高粱A_2类型CMS育性恢复基因的定位研究  

Study of the Locum of Fertility Restorer Gene of A_2 CMS in Sorghum bicolor L Meonch

作　　者：赵威军[1] 仪治本[2] 孙毅 梁小红[2] 李金梅[1] 

机构地区：[1]山西省农业科学院高粱研究所,山西晋中030600 [2]中北大学,山西太原030051 [3]山西省农业生物技术研究中心,山西太原030031

出　　处：《华北农学报》2006年第4期17-21,共5页Acta Agriculturae Boreali-Sinica

基　　金：山西省科技攻关项目(021011)

摘　　要：以2个高粱A2类型雄性不育系及相应的保持系(A2V4/B2V4,A2TX622/B2TX622),2个恢复系(1383_2,晋粱5号)和3个组合(A2V4×1383_2,A2V4×晋粱5号,A2TX622×晋粱5号)的F1,F2群体为材料,采用SSR标记方法,分析A2类型雄性不育的育性恢复基因。结果发现,只有标记Xtxp65在A2TX622×晋粱5号的F2群体中与育性出现共分离现象,育性恢复基因与标记间距离为3.4 cM,位于连锁群J上;同样只有标记Xtxp141在A2V4×晋粱5号的F2群体中与育性出现共分离现象,该标记与育性恢复基因间的距离为13.4 cM,位于连锁群G上;在A2V4×1383_2的F2群体中,标记与育性未出现共分离现象。这说明,与这2个标记连锁的A2CMS育性恢复基因是位于同一个恢复系(晋粱5号)中的2个独立的位点;在A2恢复系材料中,至少有3个独立遗传的不同位点与A2雄性不育性的恢复有关。In this study we investigated the locum of fertility restorer gene of A2 cytoplasmic male sterility（CMS） in Sorghum with two cytoplasmic male sterile lines and maintainer lines（A2V4/B2V4, A2TX622/B2TX622）, two restorers （1383-2, Jinliang 5） and F1 and F2 populations of three cross classifications（A2V4 × 1383-2, A2V4 × Jinliang 5 and A2TX622 × Jinliang 5） by simple sequence repeat（SSR） technique. The results demonstrated that only one SSR marker, Xtxt65 was found to link with fertility restorer gene in F2 population of A2Tx622 × Jinliang 5 and located in linkage groups （LG） J,the map distance between them was 3.4 cM;only one SSR marker, Xtxtl41 was found to link with fertility restorer gene in F2 population of A2V4 × Jinliang 5 and located in LG G,the map distance between them was 13.4 cM;no SSR marker was found to link with fertility restorer gene in F2 population of A2V4 × 1383-2. This showed that the fertility restorer genes linked with these two SSR marker were located two independent loci of one restore Jinliang 5, and at least three independent loci in restorers were associated with A2 CMS restoration.

关 键 词：高粱 CMS A2 SSR 育性恢复基因 

分 类 号：S514.03[农业科学—作物学]