羟基红花黄色素A缓解大鼠心肌线粒体损伤的作用研究  

Inhibitory Effect of Hydroxysafflor Yellow A Against Rat Myocardial Mitochondrial Injury

作　　者：吴伟[1] 李金荣[1] 朴永哲[1] 董宁宁[1] 金鸣[1] 

机构地区：[1]首都医科大学附属北京安贞医院北京市心肺血管疾病研究所,北京100029

出　　处：《中国药学杂志》2006年第16期1225-1227,共3页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30171146)

摘　　要：目的研究红花成分羟基红花黄色素A(HSYA)对大鼠心肌线粒体损伤的作用。方法采用低温离心法制备大鼠心肌线粒体;比浊法检测线粒体肿胀;荧光偏振法测量线粒体膜流动性;硫代巴比妥酸比色法观察线粒体脂质过氧化水平。结果HSYA可明显减轻离体大鼠心肌线粒体肿胀(P<0.05)、缓解线粒体膜流动性的下降(P<0.001)、抑制羟自由基诱导的线粒体脂质过氧化(P<0.001)。结论HSYA可减轻大鼠心肌线粒体的损伤。OBJECTIVE To investigate the inhibitory effects of hydroxysafflor yellow A （HSYA） against rat myocardial mitoehondrial injury. METHODS Rat myocardial mitochondria were prepared through centrifugation procedure; mitochondrial swelling was observed by turbidity assay. Mitochondrial membrane fluidity was determined by fluorescence polarization technique. The elevated malonyldialdehyde （MDA） in mitochondria induced by lipid peroxidation was observed by colorimetry. RESULTS Rat myocardial mitochondrial swelling, mitochondrial membrane fluidity decrease, and the mitochondria MDA elevation were remitted apparently （ P 〈 0.05） by HSYA treatment. CONCLUSION It is suggested tbat HSYA is effective in inhibiting rat myocardial mitochondrial injury.

关 键 词：羟基红花黄色素A 线粒体肿胀 线粒体膜流动性 

分 类 号：R965[医药卫生—药理学] R972[医药卫生—药学]