LC-MS鉴定家兔口服血府逐瘀汤提取物后体液中的成分  

Identification of Constituents of Xuefu Zhuyu Tang in Rabbit Body Fluids by Liquid Chromatography-Electrospray Mass Spectrometry

作　　者：栾连军[1] 胡柳[1] 裘国丽[1] 程翼宇[1] 

机构地区：[1]浙江大学中药科学与工程学系,杭州310027

出　　处：《中国药学杂志》2006年第16期1252-1255,共4页Chinese Pharmaceutical Journal

基　　金：国家重点基础研究发展规划(973计划)项目(G1999054405);浙江省中医药重点开发项目(2003KF004)

摘　　要：目的鉴定兔血浆及尿液中吸收的复方成分及其代谢物,以阐明复方的有效成分。方法家兔口服精制血府逐瘀方提取物后收集血液和尿液样本;采用固相萃取技术进行样品预处理,LC/MS联用一级全扫描质谱分析,以对照品为参照,鉴定血液及尿液中的吸收成分及其代谢物。结果血样中检测到8个原型化合物,尿样中检测到7个原型化合物,同时,确定了尿样中3个葡醛酸化代谢物。结论研究结果进一步阐明了该方的药效成分,为开展药动学研究奠定了实验基础。OBJECTIE To identify the effective eonstituents and metabolites of refined Xuefu Zhuyu Tang in rabbit plasma and urine. METHODS Blood and urine samples were collected aider oral administration of the relined Xuefu Zhuyu Tang to rabbits, and samples purification were carried out by solid phase extraction（SPE） with EWVI-C18 cartridges and analyzed by LC-ESI-MS. RESULTS Eight and seven compounds were identified in blood and urine, respectively, and three galcuronides were also detected in urine. CONCLUSION The results further clarify the effective constituents and found the experimental basis to the researeh of pharmacokinetics.

关 键 词：高效液相色谱-质谱联用 体内药物分析 精制血府逐瘀方 

分 类 号：R917[医药卫生—药物分析学]