内皮型一氧化氮合酶基因G894T和G蛋白β3亚单位基因C825T多态性对高血压的协同作用  

Synergistic Effects between eNOS Gene G894T and GNB3 Gene C825T Polymorphisms in Essential Hypertension

作　　者：李东宝[1] 华琦[1] 皮林[1] 

机构地区：[1]首都医科大学宣武医院心血管内科

出　　处：《首都医科大学学报》2006年第4期480-484,共5页Journal of Capital Medical University

基　　金：首都医学发展基金(2002-2007)资助项目

摘　　要：目的探讨内皮型一氧化氮合酶(eNOS)基因G894T和G蛋白β3亚单位(GBN3)基因C825T多态性对原发性高血压(EH)的协同作用。方法采用多聚酶链式反应结合限制性内切酶片段长度多态分析方法,检测310例健康人和151例高血压患者的eNOS基因G894T多态性和GBN3基因C825T多态性。结果EH组eNOS G894T多态性的GT+TT基因型和T等位基因频率显著高于对照组(P<0.05);EH组GNB3 C825T多态性中CC、CT、TT基因型频率、C、T等位基因频率与正常对照组比较差异无统计学意义(P>0.05);分析eNOS 894T和GNB3 825T罹患高血压的相对风险,其比数比(OR)为1.77,高于单基因eNOS 894T(0.53)和GNB3 825T(1.05)。结论eNOS基因G894T多态性的T等位基因是中国汉族人EH发病的危险因素之一。eNOS基因894T等位基因和GNB3 825T等位基因对高血压的发生具有协同作用。Objective To investigate the interaction between the G894T polymorphism of endothelial nitric oxide synthase （eNOS） gene and the C825T polymorphism of GNB3 in the way of sunergistic effects for essential hypertension （EH）. Methods Polymerase chain reaction combined with restriction enzyme digestion was used to detect the G894T polymorphism of eNOS gene and C825T polymorphism of GNB3 gene in 151 normotensive controls and 310 hypertensive patients. Results The frequencies of T allele and GT＋TT genotype of eNOS gene G894T polymorphism in EH were significantly higher than in control group; There were no significant difference of the GNB3 gene C825T polymorphism CC, CT, TT genotypes and C, Talleles between hypertensive patients and normotensive controls; The odds ratio（OR） estimated by combined analysis with eNOS 894T and GNB3 825T （1.77） was markedly increased compared with that estimated alone from either eNOS 894T allele （0.53） or GNB3 825T allele（1.05）. Conclusion eNOS 894T allele should be a risk factor for EH in chinese Han nationality,there was a significant synergistic effects of eNOS 894T allele and GNB3 825T allele in EH.

关 键 词：基因多态性 原发性高血压 协同作用 G蛋白 内皮型一氧化氮合酶 

分 类 号：R541.3[医药卫生—心血管疾病]