莪术、三棱和白介素-6对人乳腺癌细胞凋亡的诱导作用  

Effect of Rhizoma Curcumate Zedoariae,Herbra Trifolii Repentis and IL-6-induced on Apoptosis of Human Breast Cancer Cell Line MCF-7

作　　者：张瑾峰[1] 王喆[1] 刘欣[1] 唐佐青[1] 谢文杰[1] 付桂芳[1] 

机构地区：[1]首都医科大学细胞与遗传学教研室

出　　处：《首都医科大学学报》2006年第4期492-493,共2页Journal of Capital Medical University

基　　金：北京市青年科技骨干培养基金资助项目

摘　　要：目的探讨莪术、三棱以及白细胞介素-6(IL-6)对人乳腺癌细胞(MCF-7)凋亡的诱导作用。方法实验共设IL-6、莪术提取液与三棱提取液单独作用组、莪术与三棱协同作用组以及莪术、三棱分别与IL-6合并用药组,用EPICS-ELITE型流式细胞仪观察莪术、三棱和IL-6各自及协同作用对体外传代培养MCF-7细胞凋亡的影响。结果莪术、三棱I、L-6对MCF-7细胞的凋亡有诱导作用,莪术与三棱共同作用效果明显高于各自单独作用,与IL-6合并用药显著增强诱导凋亡作用。结论诱导肿瘤细胞的凋亡可能是莪术、三棱抑癌作用机制之一。Objective To explore the mechanism of inhibitory effects on breast cancer cell （MCF-7） of two Chinese traditional herbs （rhizoma curcumate zedoariae and herbra trifolii repentis） and IL-6. Methods Human breast cancer cell line MCF-7 was adopted as target cell. Tumor cell apoptosis was investigated by means of flow cytometry. Results The extracts of both herbs induced apoptosis in MCF-7 cells. The combination of two herbs had stronger apoptosis-inducing effect than either alone. The presence of these two herbs enhanced IL-6-induced apoptosis. Conclusion Two herbs,Rhizoma curcumate zedoariae and herbra trifolii repentis induce apoptosis in breast cancer cell line MCF-7. This may be one of the mechanisms for anticancer properties of these herbs.

关 键 词：莪术 三棱 白细胞介素-6 人乳腺癌细胞 凋亡 

分 类 号：Q279[生物学—细胞生物学]