微囊化兔坐骨神经组织移植对大鼠脊髓损伤神经元的影响  

Influence of transplanting microencapsulated rabbit sciatic nerve tissue on spinal cord injured neurons in adult rats

作　　者：万斌[1] 马建敏[1] 傅文学[2] 刘德明[1] 

机构地区：[1]南昌大学医学院解剖学教研室,南昌330006 [2]九江学院医学院解剖教研室,九江332000

出　　处：《解剖学杂志》2006年第4期463-467,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30060034);江西省自然科学基金(0140126)

摘　　要：目的：观察大鼠脊髓半横断损伤后植入微囊化兔坐骨神经组织对一氧化氮合酶(NOS)表达的影响。方法：尼氏染色和NADPH-d组织化学染色。利用图像分析系统检测染色标记细胞数和阳性细胞平均面积、平均光密度。结果：脊髓损伤后神经元数量减少,尼氏体脱失、溶解。术后3d,微囊组NOS阳性细胞数、阳性细胞平均面积及平均光密度均明显高于单损组；术后7d微囊组NOS阳性细胞数和阳性细胞平均面积较细胞组高；术后14d,细胞组NOS阳性细胞数急剧增高超过微囊组,但微囊组仍平稳上升。结论：微囊化兔坐骨神经组织移植能诱导早期NOS表达增加及抑制后期NO过量产生,减轻脊髓继发性损伤,有利于损伤脊髓的修复和再生。Objective： To observe the influence of the NOS expression on the spinal cord semi-transection injury in adult rats after transplantation of the microencapsulated rabbit sciatic nerve tissue. Methods： A total of 90 adult Sprague-Dawley（SD） rats were randomly divided into 3 groups and were studied with Nissl staining and NADPH- d histochemistry. The staining results were analyzed with the image analysis system. Results： After spinal cord injury, the number of the neurons was reduced and the Nissl substance was lost or dissolved. On the 14th day, the number of the Nissl stained neurons increased remarkably and their volume was enhanced. These changes became most evident in group implanted with microencapsulated nerves tissue. NOS positive reaction in microencapsulated nerves tissue implanted group and nerves tissue implanted group was superior to that in non-implanted group on the 3rd day after injury. On the 14th day, cell number with NOS positive reaction in nerves tissue implanted group increased rapidly, while that in microencapsulated nerves tissue implanted group showed a steady increasing tendency. Conclusion： Xenotransplantation of microencapsulated sciatic nerve tissue/cells can promote NOS expression in the earlier period, inhibit NO over dose creations anaphase, and improve the functional recovery, which is helpful for the repair and the regeneration of the injured spinal cord.

关 键 词：微囊 坐骨神经 移植 异种 脊髓损伤 神经元 

分 类 号：R651.2[医药卫生—外科学] R338.64[医药卫生—临床医学]